Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging

Near-infrared gold-doped CdHgTe quantum dots (QDs) with improved photoluminescence and biocompatibility were developed using an aqueous solution route with l -glutathione and l -cysteine as stabilizers. As-prepared Au:CdHgTe QDs were covalently linked to arginine–glycine–aspartic acid (RGD) peptide,...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2012-05, Vol.403 (5), p.1343-1352
Main Authors: Han, Sihai, Mu, Ying, Zhu, Qiangyuan, Gao, Yibo, Li, Zuhong, Jin, Qinhan, Jin, Wei
Format: Article
Language:English
Subjects:
Analytical Chemistry
Animals
Aspartate
Biochemistry
Biocompatibility
Biomedical materials
Cadmium Compounds - chemistry
Cell Line, Tumor
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Cysteine
Female
Fluorescence
Food Science
Humans
Imaging
In vivo testing
In vivo tests
Laboratory Medicine
Male
Markers
Mercury Compounds - chemistry
Mice
Mice, Inbred ICR
Molecular Imaging - methods
Monitoring/Environmental Analysis
Monoclonal antibodies
Neoplasms - chemistry
Neoplasms - diagnosis
Original Paper
Photoluminescence
Quantum Dots
Surgical implants
Tumors
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Summary:Near-infrared gold-doped CdHgTe quantum dots (QDs) with improved photoluminescence and biocompatibility were developed using an aqueous solution route with l -glutathione and l -cysteine as stabilizers. As-prepared Au:CdHgTe QDs were covalently linked to arginine–glycine–aspartic acid (RGD) peptide, anti-epidermal growth factor receptor (EGFR) monoclonal antibody (MAb), and anti- carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) MAb separately. Three Au:CdHgTe QD bioconjugates (QD800-RGD, QD820-anti-CEACAM1, and QD840-anti-EGFR) were successfully used as probes for in vivo tumor-targeted multispectral fluorescence imaging of xenografts. Fluorescence signals from the QD bioconjugates used to detect three tumor markers were spectrally unmixed, and their co-localization was analyzed. The results indicate that multiple tumor markers could be simultaneously detected by multispectral fluorescence imaging in vivo using QD bioconjugates as probes. This approach has excellent potential as an imaging method for the noninvasive exploration and detection of multiple tumor markers in vivo, thereby substantially aiding the diagnosis of cancer. Figure In vivo tumor-targeted multispectral fluorescence imaging with Au:CdHgTe quantum dots
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-012-5921-y