Nicotiana tabacum actin-depolymerizing factor 2 is involved in actin-driven, auxin-dependent patterning

Polar transport of auxin has been identified as a central element of pattern formation. To address the underlying cellular mechanisms, we use the tobacco cell line (Nicotiana tabacum L. cv. Bright Yellow 2; BY-2) as model. We showed previously that cell divisions within a cell file are synchronized...

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Bibliographic Details
Published in:Journal of plant physiology 2013-08, Vol.170 (12), p.1057-1066
Main Authors: Durst, Steffen, Nick, Peter, Maisch, Jan
Format: Article
Language:English
Subjects:
Actin
Actin-binding proteins
Actins - metabolism
Auxin signalling
auxins
Biotechnology
Cell division
Cell division pattern
Cellular
Destrin - metabolism
Division
Filaments
Indoleacetic Acids - metabolism
Mathematical models
Microfilament Proteins - metabolism
microfilaments
Nicotiana - metabolism
Nicotiana tabacum
Physiology
Plant Proteins - metabolism
Signalling
Tobacco BY-2
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Summary:Polar transport of auxin has been identified as a central element of pattern formation. To address the underlying cellular mechanisms, we use the tobacco cell line (Nicotiana tabacum L. cv. Bright Yellow 2; BY-2) as model. We showed previously that cell divisions within a cell file are synchronized by polar auxin flow, linked to the organization of actin filaments (AF) which, in turn, is modified via actin-binding proteins (ABPs). From a preparatory study for disturbed division synchrony in cell lines overexpressing different ABPs, we identified the actin depolymerizing factor 2 (ADF2). A cell line overexpressing GFP-NtADF2 was specifically affected in division synchrony. The cell division pattern could be rescued by addition of Phosphatidylinositol 4,5-bisphosphate (PIP2) or by phalloidin. These observations allow to draw first conclusions on the pathway linking auxin signalling via actin reorganization to synchronized cell division placing the regulation of cortical actin turnover by ADF2 into the focus.
ISSN:0176-1617
1618-1328
DOI:10.1016/j.jplph.2013.03.002