Fluorescent proteins as a toolkit for in vivo imaging

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria, and its mutant variants, are the only fully genetically encoded fluorescent probes available and they have proved to be excellent tools for labeling living specimens. Since 1999, numerous GFP homologues have been discovered in Ant...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2005-12, Vol.23 (12), p.605-613
Main Authors: Chudakov, Dmitriy M., Lukyanov, Sergey, Lukyanov, Konstantin A.
Format: Article
Language:English
Subjects:
Aequorea victoria
Anthozoa
Biosensing Techniques - methods
Cloning
Color
Copepoda
Evolution, Molecular
Fluorescence microscopy
Fluorescence Resonance Energy Transfer
Gene Expression
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Hydrozoa
Labeling
Light
Luminescent Proteins - analysis
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Molecular Biology - methods
Motility
Organisms
Photochemistry - methods
Phylogenetics
Protein Interaction Mapping - methods
Proteins
Proteins - analysis
Proteins - chemistry
Recombinant Proteins - analysis
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
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Summary:Green fluorescent protein (GFP) from the jellyfish Aequorea victoria, and its mutant variants, are the only fully genetically encoded fluorescent probes available and they have proved to be excellent tools for labeling living specimens. Since 1999, numerous GFP homologues have been discovered in Anthozoa, Hydrozoa and Copepoda species, demonstrating the broad evolutionary and spectral diversity of this protein family. Mutagenic studies gave rise to diversified and optimized variants of fluorescent proteins, which have never been encountered in nature. This article gives an overview of the GFP-like proteins developed to date and their most common applications to study living specimens using fluorescence microscopy.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2005.10.005