Separation of fructooligosaccharides on a cation-exchange HPLC column in silver form with refractometric detection

An isocratic HPLC method was developed for the analysis of fructooligosaccharides in mixtures with sucrose, glucose and fructose. Fructooligosaccharides and sucrose were extensively hydrolyzed during the analyses at 80 degree C but at 40 degree C there was a negligible hydrolysis with a peak resolut...

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Bibliographic Details
Published in:Biotechnology techniques 1999, Vol.13 (12), p.889-892
Main Authors: Antosova, M, Polakovic, M, Bales, V
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
food composition
food quality
fructooligosaccharides
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
saccharides
silver
Various methods and equipments
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Summary:An isocratic HPLC method was developed for the analysis of fructooligosaccharides in mixtures with sucrose, glucose and fructose. Fructooligosaccharides and sucrose were extensively hydrolyzed during the analyses at 80 degree C but at 40 degree C there was a negligible hydrolysis with a peak resolution over 1.5 for every pair of subsequently eluting saccharides:.
ISSN:0951-208X
1573-6784
DOI:10.1023/A:1008986426849