Bovine milk lactoferrin modulates the proliferative lymphocyte response to phytohemagglutinin

Bovine milk lactoferrin (2 to 20 μg ml⁻¹) changed enhancement of [³H]thymidine incorporation by phytohemagglutinin-stimulated rat spleen lymphocytes into suppression as their lactoferrin-withdrawal incorporation increased to greater than 10 000 cpm culture⁻¹ under the present isotope-labeling condit...

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Bibliographic Details
Published in:Biotechnology letters 1999-12, Vol.21 (12), p.1083-1089
Main Authors: Yabe, Noritsugu, Itagaki, Yasuharu, Tanimoto, Morimasa, Matsui, Hisao
Format: Article
Language:English
Subjects:
12-O-^Atetradecanoylphorbol-13-acetate
Biological and medical sciences
Cattle
Cell physiology
Fundamental and applied biological sciences. Psychology
ionomycin
isotope labeling
lactoferrin
lectins
lymphocyte proliferation
Lymphocytes
Milk
Molecular and cellular biology
phytohemagglutinin
rats
Responses to growth factors, tumor promotors, other factors
spleen
thymidine
tritium
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Summary:Bovine milk lactoferrin (2 to 20 μg ml⁻¹) changed enhancement of [³H]thymidine incorporation by phytohemagglutinin-stimulated rat spleen lymphocytes into suppression as their lactoferrin-withdrawal incorporation increased to greater than 10 000 cpm culture⁻¹ under the present isotope-labeling conditions. The enhancement disappeared by 15-min delayed addition of lactoferrin after addition of lectin. There was no lactoferrin effect when the cells were stimulated with 12-O-tetradecanoylphorbol-13-acetate plus ionomycin. Thus, lactoferrin has a certain extracellular effect on lymphocyte proliferation in response to the lectin.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005696508289