Loading…

Rapid detection of B2-ST131 clonal group of extended-spectrum β-lactamase–producing Escherichia coli by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry: discovery of a peculiar amino acid substitution in B2-ST131 clonal group

Abstract One reason for the spread of extended-spectrum β-lactamase (ESBL)–producing Escherichia coli worldwide is the global pandemic of the B2-ST131 clonal group. We searched for the specific biomarker peaks to distinguish between the B2-ST131 clonal group and other sequence type (ST) clonal group...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2015-11, Vol.83 (3), p.237-244
Main Authors: Nakamura, Akihiro, Komatsu, Masaru, Kondo, Akira, Ohno, Yuki, Kohno, Hisashi, Nakamura, Fumihiko, Matsuo, Shuji, Ohnuma, Kenichiro, Hatano, Naoya, Kawano, Seiji
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c622t-947983ce89becc2baa3a9884403e08848d0cc6ea8a64cee21d583b1ab720a1093
cites cdi_FETCH-LOGICAL-c622t-947983ce89becc2baa3a9884403e08848d0cc6ea8a64cee21d583b1ab720a1093
container_end_page 244
container_issue 3
container_start_page 237
container_title Diagnostic microbiology and infectious disease
container_volume 83
creator Nakamura, Akihiro
Komatsu, Masaru
Kondo, Akira
Ohno, Yuki
Kohno, Hisashi
Nakamura, Fumihiko
Matsuo, Shuji
Ohnuma, Kenichiro
Hatano, Naoya
Kawano, Seiji
description Abstract One reason for the spread of extended-spectrum β-lactamase (ESBL)–producing Escherichia coli worldwide is the global pandemic of the B2-ST131 clonal group. We searched for the specific biomarker peaks to distinguish between the B2-ST131 clonal group and other sequence type (ST) clonal groups isolated from clinical specimens obtained in our hospital. Biomarker peaks at m / z 7650 in the B2-ST131 group (sensitivity of 100% and specificity of 89.7%) and m / z 7707 in the other ST clonal groups showed the highest discrimination abilities. We further verified reproducibility against other Japanese clinical isolates obtained in another area of Japan. Differences between the molecular mass at the 7650 m / z and 7707 m / z peaks indicated an E34A amino acid substitution by proteomic and genomic analysis. Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry rapidly and simply identified the B2-ST131 clonal group in routine examinations and will allow for adequate empirical therapy and the possibility to control both hospital infections and the global pandemic.
doi_str_mv 10.1016/j.diagmicrobio.2015.06.024
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1746875261</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0732889315002874</els_id><sourcerecordid>1721918091</sourcerecordid><originalsourceid>FETCH-LOGICAL-c622t-947983ce89becc2baa3a9884403e08848d0cc6ea8a64cee21d583b1ab720a1093</originalsourceid><addsrcrecordid>eNqNUkuO1DAQDT8xzcAVkMWKTRrbSSfOLJBgGD7SSEjMsLYqdnV3NUncYzujaVbcgZtwEA7BSXDoASHEYhZWWar36vmVX5Y9EXwuuKiebeaWYNWT8a4lN5dcLOa8mnNZ3s5mQtVNznnN72YzXhcyV6opDrIHIWw4F7Ip-f3sQFaFqEpezm7d-QBbssxiRBPJDcwt2UuZn52LQjDTuQE6tvJu3E4NvIo4WLR52Ca0H3v2_VvegYnQQ8AfX75uvbOjoWHFToJZoyezJmDGdcTaHesherrKIQQKES3rEskn6eD89pd2OvQZpmuaFanH3C3zZUerdUzkENhe1_UY_e6IWQrGXaLfTW8DlnpjR-AZ9DQ4Bib5CmMbIsVxP374v7WH2b0ldAEfXdfD7OPrk_Pjt_np-zfvjl-c5qaSMuZNWTeqMKiaFo2RLUABjVJlyQvkqSrLjakQFFSlQZTCLlTRCmhryUHwpjjMnu7npjVdjBii7pMD7DoY0I1Bi7qsVL2QlbgBVIpGKN5M0KM9NMUhBI9LvfXUg99pwfWUF73Rf-dFT3nRvNIpL4n8-FpnbHu0f6i_A5IAr_YATIu5JPQ6GMLBoCWf_kJbRzfTef7PGNPRQAa6T7jDsHGjT9-RfOkgNddnU3Kn4IoF51LVZfETFyb06A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1721918091</pqid></control><display><type>article</type><title>Rapid detection of B2-ST131 clonal group of extended-spectrum β-lactamase–producing Escherichia coli by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry: discovery of a peculiar amino acid substitution in B2-ST131 clonal group</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Nakamura, Akihiro ; Komatsu, Masaru ; Kondo, Akira ; Ohno, Yuki ; Kohno, Hisashi ; Nakamura, Fumihiko ; Matsuo, Shuji ; Ohnuma, Kenichiro ; Hatano, Naoya ; Kawano, Seiji</creator><creatorcontrib>Nakamura, Akihiro ; Komatsu, Masaru ; Kondo, Akira ; Ohno, Yuki ; Kohno, Hisashi ; Nakamura, Fumihiko ; Matsuo, Shuji ; Ohnuma, Kenichiro ; Hatano, Naoya ; Kawano, Seiji</creatorcontrib><description>Abstract One reason for the spread of extended-spectrum β-lactamase (ESBL)–producing Escherichia coli worldwide is the global pandemic of the B2-ST131 clonal group. We searched for the specific biomarker peaks to distinguish between the B2-ST131 clonal group and other sequence type (ST) clonal groups isolated from clinical specimens obtained in our hospital. Biomarker peaks at m / z 7650 in the B2-ST131 group (sensitivity of 100% and specificity of 89.7%) and m / z 7707 in the other ST clonal groups showed the highest discrimination abilities. We further verified reproducibility against other Japanese clinical isolates obtained in another area of Japan. Differences between the molecular mass at the 7650 m / z and 7707 m / z peaks indicated an E34A amino acid substitution by proteomic and genomic analysis. Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry rapidly and simply identified the B2-ST131 clonal group in routine examinations and will allow for adequate empirical therapy and the possibility to control both hospital infections and the global pandemic.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2015.06.024</identifier><identifier>PMID: 26316404</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Substitution ; B2-ST131 ; Bacteriological Techniques - methods ; beta-Lactamases - chemistry ; beta-Lactamases - secretion ; ESBL-producing Escherichia coli ; Escherichia coli ; Escherichia coli - chemistry ; Escherichia coli - enzymology ; Escherichia coli - isolation &amp; purification ; Genomic analysis ; Humans ; Infectious Disease ; Internal Medicine ; Japan ; MALDI-TOF MS ; Proteome - analysis ; Proteomic analysis ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Time Factors ; YahO protein</subject><ispartof>Diagnostic microbiology and infectious disease, 2015-11, Vol.83 (3), p.237-244</ispartof><rights>Elsevier Inc.</rights><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c622t-947983ce89becc2baa3a9884403e08848d0cc6ea8a64cee21d583b1ab720a1093</citedby><cites>FETCH-LOGICAL-c622t-947983ce89becc2baa3a9884403e08848d0cc6ea8a64cee21d583b1ab720a1093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26316404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakamura, Akihiro</creatorcontrib><creatorcontrib>Komatsu, Masaru</creatorcontrib><creatorcontrib>Kondo, Akira</creatorcontrib><creatorcontrib>Ohno, Yuki</creatorcontrib><creatorcontrib>Kohno, Hisashi</creatorcontrib><creatorcontrib>Nakamura, Fumihiko</creatorcontrib><creatorcontrib>Matsuo, Shuji</creatorcontrib><creatorcontrib>Ohnuma, Kenichiro</creatorcontrib><creatorcontrib>Hatano, Naoya</creatorcontrib><creatorcontrib>Kawano, Seiji</creatorcontrib><title>Rapid detection of B2-ST131 clonal group of extended-spectrum β-lactamase–producing Escherichia coli by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry: discovery of a peculiar amino acid substitution in B2-ST131 clonal group</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract One reason for the spread of extended-spectrum β-lactamase (ESBL)–producing Escherichia coli worldwide is the global pandemic of the B2-ST131 clonal group. We searched for the specific biomarker peaks to distinguish between the B2-ST131 clonal group and other sequence type (ST) clonal groups isolated from clinical specimens obtained in our hospital. Biomarker peaks at m / z 7650 in the B2-ST131 group (sensitivity of 100% and specificity of 89.7%) and m / z 7707 in the other ST clonal groups showed the highest discrimination abilities. We further verified reproducibility against other Japanese clinical isolates obtained in another area of Japan. Differences between the molecular mass at the 7650 m / z and 7707 m / z peaks indicated an E34A amino acid substitution by proteomic and genomic analysis. Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry rapidly and simply identified the B2-ST131 clonal group in routine examinations and will allow for adequate empirical therapy and the possibility to control both hospital infections and the global pandemic.</description><subject>Amino Acid Substitution</subject><subject>B2-ST131</subject><subject>Bacteriological Techniques - methods</subject><subject>beta-Lactamases - chemistry</subject><subject>beta-Lactamases - secretion</subject><subject>ESBL-producing Escherichia coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - isolation &amp; purification</subject><subject>Genomic analysis</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Internal Medicine</subject><subject>Japan</subject><subject>MALDI-TOF MS</subject><subject>Proteome - analysis</subject><subject>Proteomic analysis</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Time Factors</subject><subject>YahO protein</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNUkuO1DAQDT8xzcAVkMWKTRrbSSfOLJBgGD7SSEjMsLYqdnV3NUncYzujaVbcgZtwEA7BSXDoASHEYhZWWar36vmVX5Y9EXwuuKiebeaWYNWT8a4lN5dcLOa8mnNZ3s5mQtVNznnN72YzXhcyV6opDrIHIWw4F7Ip-f3sQFaFqEpezm7d-QBbssxiRBPJDcwt2UuZn52LQjDTuQE6tvJu3E4NvIo4WLR52Ca0H3v2_VvegYnQQ8AfX75uvbOjoWHFToJZoyezJmDGdcTaHesherrKIQQKES3rEskn6eD89pd2OvQZpmuaFanH3C3zZUerdUzkENhe1_UY_e6IWQrGXaLfTW8DlnpjR-AZ9DQ4Bib5CmMbIsVxP374v7WH2b0ldAEfXdfD7OPrk_Pjt_np-zfvjl-c5qaSMuZNWTeqMKiaFo2RLUABjVJlyQvkqSrLjakQFFSlQZTCLlTRCmhryUHwpjjMnu7npjVdjBii7pMD7DoY0I1Bi7qsVL2QlbgBVIpGKN5M0KM9NMUhBI9LvfXUg99pwfWUF73Rf-dFT3nRvNIpL4n8-FpnbHu0f6i_A5IAr_YATIu5JPQ6GMLBoCWf_kJbRzfTef7PGNPRQAa6T7jDsHGjT9-RfOkgNddnU3Kn4IoF51LVZfETFyb06A</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Nakamura, Akihiro</creator><creator>Komatsu, Masaru</creator><creator>Kondo, Akira</creator><creator>Ohno, Yuki</creator><creator>Kohno, Hisashi</creator><creator>Nakamura, Fumihiko</creator><creator>Matsuo, Shuji</creator><creator>Ohnuma, Kenichiro</creator><creator>Hatano, Naoya</creator><creator>Kawano, Seiji</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20151101</creationdate><title>Rapid detection of B2-ST131 clonal group of extended-spectrum β-lactamase–producing Escherichia coli by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry: discovery of a peculiar amino acid substitution in B2-ST131 clonal group</title><author>Nakamura, Akihiro ; Komatsu, Masaru ; Kondo, Akira ; Ohno, Yuki ; Kohno, Hisashi ; Nakamura, Fumihiko ; Matsuo, Shuji ; Ohnuma, Kenichiro ; Hatano, Naoya ; Kawano, Seiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c622t-947983ce89becc2baa3a9884403e08848d0cc6ea8a64cee21d583b1ab720a1093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amino Acid Substitution</topic><topic>B2-ST131</topic><topic>Bacteriological Techniques - methods</topic><topic>beta-Lactamases - chemistry</topic><topic>beta-Lactamases - secretion</topic><topic>ESBL-producing Escherichia coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - isolation &amp; purification</topic><topic>Genomic analysis</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Internal Medicine</topic><topic>Japan</topic><topic>MALDI-TOF MS</topic><topic>Proteome - analysis</topic><topic>Proteomic analysis</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Time Factors</topic><topic>YahO protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakamura, Akihiro</creatorcontrib><creatorcontrib>Komatsu, Masaru</creatorcontrib><creatorcontrib>Kondo, Akira</creatorcontrib><creatorcontrib>Ohno, Yuki</creatorcontrib><creatorcontrib>Kohno, Hisashi</creatorcontrib><creatorcontrib>Nakamura, Fumihiko</creatorcontrib><creatorcontrib>Matsuo, Shuji</creatorcontrib><creatorcontrib>Ohnuma, Kenichiro</creatorcontrib><creatorcontrib>Hatano, Naoya</creatorcontrib><creatorcontrib>Kawano, Seiji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakamura, Akihiro</au><au>Komatsu, Masaru</au><au>Kondo, Akira</au><au>Ohno, Yuki</au><au>Kohno, Hisashi</au><au>Nakamura, Fumihiko</au><au>Matsuo, Shuji</au><au>Ohnuma, Kenichiro</au><au>Hatano, Naoya</au><au>Kawano, Seiji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of B2-ST131 clonal group of extended-spectrum β-lactamase–producing Escherichia coli by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry: discovery of a peculiar amino acid substitution in B2-ST131 clonal group</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>83</volume><issue>3</issue><spage>237</spage><epage>244</epage><pages>237-244</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>Abstract One reason for the spread of extended-spectrum β-lactamase (ESBL)–producing Escherichia coli worldwide is the global pandemic of the B2-ST131 clonal group. We searched for the specific biomarker peaks to distinguish between the B2-ST131 clonal group and other sequence type (ST) clonal groups isolated from clinical specimens obtained in our hospital. Biomarker peaks at m / z 7650 in the B2-ST131 group (sensitivity of 100% and specificity of 89.7%) and m / z 7707 in the other ST clonal groups showed the highest discrimination abilities. We further verified reproducibility against other Japanese clinical isolates obtained in another area of Japan. Differences between the molecular mass at the 7650 m / z and 7707 m / z peaks indicated an E34A amino acid substitution by proteomic and genomic analysis. Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry rapidly and simply identified the B2-ST131 clonal group in routine examinations and will allow for adequate empirical therapy and the possibility to control both hospital infections and the global pandemic.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26316404</pmid><doi>10.1016/j.diagmicrobio.2015.06.024</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0732-8893
ispartof Diagnostic microbiology and infectious disease, 2015-11, Vol.83 (3), p.237-244
issn 0732-8893
1879-0070
language eng
recordid cdi_proquest_miscellaneous_1746875261
source ScienceDirect Freedom Collection 2022-2024
subjects Amino Acid Substitution
B2-ST131
Bacteriological Techniques - methods
beta-Lactamases - chemistry
beta-Lactamases - secretion
ESBL-producing Escherichia coli
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - enzymology
Escherichia coli - isolation & purification
Genomic analysis
Humans
Infectious Disease
Internal Medicine
Japan
MALDI-TOF MS
Proteome - analysis
Proteomic analysis
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Time Factors
YahO protein
title Rapid detection of B2-ST131 clonal group of extended-spectrum β-lactamase–producing Escherichia coli by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry: discovery of a peculiar amino acid substitution in B2-ST131 clonal group
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T19%3A08%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20detection%20of%20B2-ST131%20clonal%20group%20of%20extended-spectrum%20%CE%B2-lactamase%E2%80%93producing%20Escherichia%20coli%20by%20matrix-assisted%20laser%20desorption%20ionization%E2%80%93time-of-flight%20mass%20spectrometry:%20discovery%20of%20a%20peculiar%20amino%20acid%20substitution%20in%20B2-ST131%20clonal%20group&rft.jtitle=Diagnostic%20microbiology%20and%20infectious%20disease&rft.au=Nakamura,%20Akihiro&rft.date=2015-11-01&rft.volume=83&rft.issue=3&rft.spage=237&rft.epage=244&rft.pages=237-244&rft.issn=0732-8893&rft.eissn=1879-0070&rft_id=info:doi/10.1016/j.diagmicrobio.2015.06.024&rft_dat=%3Cproquest_cross%3E1721918091%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c622t-947983ce89becc2baa3a9884403e08848d0cc6ea8a64cee21d583b1ab720a1093%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1721918091&rft_id=info:pmid/26316404&rfr_iscdi=true