Bicaudal-C plays a vital role in oogenesis in Nilaparvata lugens (Hemiptera: Delphacidae)

[Display omitted] •First characterization of Bicaudal-C gene in a non-Drosophila insect.•NlBic-C is specifically expressed in developing oocytes but not in laid eggs.•NlBic-C is required for oogenesis and oocyte maturation.•The gene is extremely highly sensitive to the RNA interference.•This gene is...

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Bibliographic Details
Published in:Journal of insect physiology 2015-08, Vol.79, p.19-26
Main Authors: Zhang, Bing-Xin, Huang, Hai-Jian, Yu, Bing, Lou, Yi-Han, Fan, Hai-Wei, Zhang, Chuan-Xi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Bicaudal-C gene
Delphacidae
Drosophila melanogaster
Female
Genes, Insect
Hemiptera
Hemiptera - genetics
Hemiptera - physiology
Male
Molecular Sequence Data
Nilaparvata lugens
Oogenesis
Organ Specificity
Phylogeny
Reproduction - genetics
Ribonucleic acid interference
RNA Interference
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Summary:[Display omitted] •First characterization of Bicaudal-C gene in a non-Drosophila insect.•NlBic-C is specifically expressed in developing oocytes but not in laid eggs.•NlBic-C is required for oogenesis and oocyte maturation.•The gene is extremely highly sensitive to the RNA interference.•This gene is a potential target in RNAi-based planthopper management. Bicaudal-C (Bic-C) was originally identified in a Drosophila melanogaster mutagenesis screen and plays vital roles in embryogenesis. In this study, we characterized the Bic-C gene in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae), an insect pest that undergoes incomplete metamorphosis. Our result showed that N. lugens Bic-C (NlBic-C) is a female-specific gene in this species. It is specifically expressed in developing oocytes and is not expressed in laid eggs. Ribonucleic acid interference (RNAi) of NlBic-C arrested the uptake of vitelline by oocytes, and resulted in undeveloped ovaries and the complete inhibition of oocyte growth in the ovarioles, suggesting that NlBic-C is required for oogenesis and oocyte maturation. NlBic-C is extremely highly sensitive to RNAi, suggesting that it may be a potential target in RNAi-based insect pest management.
ISSN:0022-1910
1879-1611
DOI:10.1016/j.jinsphys.2015.05.006