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A simple method to identify ether lipids in spermatozoa samples by MALDI-TOF mass spectrometry
Plasmalogens (alkenylacyl glycerophospholipids) are important lipid constituents of many tissues and cells (e.g., selected spermatozoa). Since the molecular weights of plasmalogens overlap with that of diacyl- or alkyl acyl lipids, sophisticated mass spectrometry (MS; including MS/MS) analysis is no...
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| Published in: | Analytical and bioanalytical chemistry 2013-08, Vol.405 (21), p.6675-6682 |
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| creator | Nimptsch, Ariane Fuchs, Beate Süß, Rosmarie Zschörnig, Kristin Jakop, Ulrike Göritz, Frank Schiller, Jürgen Müller, Karin |
| description | Plasmalogens (alkenylacyl glycerophospholipids) are important lipid constituents of many tissues and cells (e.g., selected spermatozoa). Since the molecular weights of plasmalogens overlap with that of diacyl- or alkyl acyl lipids, sophisticated mass spectrometry (MS; including MS/MS) analysis is normally used for the unequivocal identification of plasmalogens. We will show here that a simple matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (without MS/MS capability) in combination with acidic hydrolysis and subsequent derivatization with 2,4-dinitrophenylhydrazine (DNPH) and/or digestion with phospholipase A
2
(PLA
2
) is sufficient to determine the contributions of ether lipids in spermatozoa extracts. As neither diacyl nor alkylacyl lipids are sensitive to acids and do not react with DNPH, the comparison of the mass spectra before and after treatment with acids and/or DNPH addition readily provides unequivocal information about the plasmalogen content. Additionally, the released aldehydes are readily converted into the 2,4-dinitrophenylhydrazones and can be easily identified in the corresponding negative ion mass spectra. Finally, PLA
2
digestion is very useful in confirming the presence of plasmalogens. The suggested method was validated by analyzing roe deer, bovine, boar, and domestic cat spermatozoa extracts and comparing the results with isolated phospholipids.
Figure
ᅟ |
| doi_str_mv | 10.1007/s00216-013-7147-z |
| format | article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1746879978</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A344392705</galeid><sourcerecordid>A344392705</sourcerecordid><originalsourceid>FETCH-LOGICAL-c608t-d3bffbb93b07363d46bf650162b8e34fb282fc3e8a53c3018cb5024526c948683</originalsourceid><addsrcrecordid>eNqNkk9vFSEUxSdGY__oB3BjSNy4mcoFBpjlS2ttk2e6qVsJMFBpZoYR5i3e-_QyebVqY9OGBeTyOyf3JKeq3gE-AYzFp4wxAV5joLUAJurdi-oQOMia8Aa_vH8zclAd5XyLMTQS-OvqgFAJREo4rL6vUA7D1Ds0uPlH7NAcUejcOAe_RWXiEurDFLqMwojy5NKg57iLGmW9qDIyW_R1tT67rK-vztGgc14oO6dY_NL2TfXK6z67t3f3cfXt_PP16UW9vvpyebpa15ZjOdcdNd4b01KDBeW0Y9z4EgE4MdJR5g2RxFvqpG6opRikNQ0mrCHctkxySY-rj3vfKcWfG5dnNYRsXd_r0cVNViAYl6JtxXNQ3AootHgaZSAa0kIDBf3wAL2NmzSWzAq4KLtSwPQPdaN7p8Lo45y0XUzVijJGWyJwU6iT_1DldG4INo7OhzL_RwB7gU0x5-S8mlIYdNoqwGppito3RZWmqKUpalc07-8W3pjBdfeK39UoANkDuXyNNy79lehR11_dEsU-</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1670243103</pqid></control><display><type>article</type><title>A simple method to identify ether lipids in spermatozoa samples by MALDI-TOF mass spectrometry</title><source>Springer Nature</source><creator>Nimptsch, Ariane ; Fuchs, Beate ; Süß, Rosmarie ; Zschörnig, Kristin ; Jakop, Ulrike ; Göritz, Frank ; Schiller, Jürgen ; Müller, Karin</creator><creatorcontrib>Nimptsch, Ariane ; Fuchs, Beate ; Süß, Rosmarie ; Zschörnig, Kristin ; Jakop, Ulrike ; Göritz, Frank ; Schiller, Jürgen ; Müller, Karin</creatorcontrib><description>Plasmalogens (alkenylacyl glycerophospholipids) are important lipid constituents of many tissues and cells (e.g., selected spermatozoa). Since the molecular weights of plasmalogens overlap with that of diacyl- or alkyl acyl lipids, sophisticated mass spectrometry (MS; including MS/MS) analysis is normally used for the unequivocal identification of plasmalogens. We will show here that a simple matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (without MS/MS capability) in combination with acidic hydrolysis and subsequent derivatization with 2,4-dinitrophenylhydrazine (DNPH) and/or digestion with phospholipase A
2
(PLA
2
) is sufficient to determine the contributions of ether lipids in spermatozoa extracts. As neither diacyl nor alkylacyl lipids are sensitive to acids and do not react with DNPH, the comparison of the mass spectra before and after treatment with acids and/or DNPH addition readily provides unequivocal information about the plasmalogen content. Additionally, the released aldehydes are readily converted into the 2,4-dinitrophenylhydrazones and can be easily identified in the corresponding negative ion mass spectra. Finally, PLA
2
digestion is very useful in confirming the presence of plasmalogens. The suggested method was validated by analyzing roe deer, bovine, boar, and domestic cat spermatozoa extracts and comparing the results with isolated phospholipids.
Figure
ᅟ</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-013-7147-z</identifier><identifier>PMID: 23812881</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acids ; Aldehydes ; Alzheimer's disease ; Analytical Chemistry ; Animals ; Bioanalysis ; Biochemistry ; Cats ; Cattle ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Deer ; Desorption ; Digestion ; Domestic animals ; Electrochemistry ; Ether lipids ; Ethers ; Food Science ; Ionization ; Laboratory Medicine ; Lipids ; Male ; Mass spectra ; Mass spectrometry ; Mass spectroscopy ; Methods ; Monitoring/Environmental Analysis ; Physiology ; Plasmalogens - analysis ; Plasmalogens - chemistry ; Properties ; Research Paper ; Scientific imaging ; Species Specificity ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Spectroscopy ; Spermatozoa ; Spermatozoa - chemistry ; Sus scrofa ; Tandem Mass Spectrometry - methods ; Time-of-flight mass spectrometry</subject><ispartof>Analytical and bioanalytical chemistry, 2013-08, Vol.405 (21), p.6675-6682</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>COPYRIGHT 2013 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c608t-d3bffbb93b07363d46bf650162b8e34fb282fc3e8a53c3018cb5024526c948683</citedby><cites>FETCH-LOGICAL-c608t-d3bffbb93b07363d46bf650162b8e34fb282fc3e8a53c3018cb5024526c948683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23812881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nimptsch, Ariane</creatorcontrib><creatorcontrib>Fuchs, Beate</creatorcontrib><creatorcontrib>Süß, Rosmarie</creatorcontrib><creatorcontrib>Zschörnig, Kristin</creatorcontrib><creatorcontrib>Jakop, Ulrike</creatorcontrib><creatorcontrib>Göritz, Frank</creatorcontrib><creatorcontrib>Schiller, Jürgen</creatorcontrib><creatorcontrib>Müller, Karin</creatorcontrib><title>A simple method to identify ether lipids in spermatozoa samples by MALDI-TOF mass spectrometry</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Plasmalogens (alkenylacyl glycerophospholipids) are important lipid constituents of many tissues and cells (e.g., selected spermatozoa). Since the molecular weights of plasmalogens overlap with that of diacyl- or alkyl acyl lipids, sophisticated mass spectrometry (MS; including MS/MS) analysis is normally used for the unequivocal identification of plasmalogens. We will show here that a simple matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (without MS/MS capability) in combination with acidic hydrolysis and subsequent derivatization with 2,4-dinitrophenylhydrazine (DNPH) and/or digestion with phospholipase A
2
(PLA
2
) is sufficient to determine the contributions of ether lipids in spermatozoa extracts. As neither diacyl nor alkylacyl lipids are sensitive to acids and do not react with DNPH, the comparison of the mass spectra before and after treatment with acids and/or DNPH addition readily provides unequivocal information about the plasmalogen content. Additionally, the released aldehydes are readily converted into the 2,4-dinitrophenylhydrazones and can be easily identified in the corresponding negative ion mass spectra. Finally, PLA
2
digestion is very useful in confirming the presence of plasmalogens. The suggested method was validated by analyzing roe deer, bovine, boar, and domestic cat spermatozoa extracts and comparing the results with isolated phospholipids.
Figure
ᅟ</description><subject>Acids</subject><subject>Aldehydes</subject><subject>Alzheimer's disease</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Bioanalysis</subject><subject>Biochemistry</subject><subject>Cats</subject><subject>Cattle</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Deer</subject><subject>Desorption</subject><subject>Digestion</subject><subject>Domestic animals</subject><subject>Electrochemistry</subject><subject>Ether lipids</subject><subject>Ethers</subject><subject>Food Science</subject><subject>Ionization</subject><subject>Laboratory Medicine</subject><subject>Lipids</subject><subject>Male</subject><subject>Mass spectra</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Physiology</subject><subject>Plasmalogens - analysis</subject><subject>Plasmalogens - chemistry</subject><subject>Properties</subject><subject>Research Paper</subject><subject>Scientific imaging</subject><subject>Species Specificity</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Spectroscopy</subject><subject>Spermatozoa</subject><subject>Spermatozoa - chemistry</subject><subject>Sus scrofa</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Time-of-flight mass spectrometry</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNkk9vFSEUxSdGY__oB3BjSNy4mcoFBpjlS2ttk2e6qVsJMFBpZoYR5i3e-_QyebVqY9OGBeTyOyf3JKeq3gE-AYzFp4wxAV5joLUAJurdi-oQOMia8Aa_vH8zclAd5XyLMTQS-OvqgFAJREo4rL6vUA7D1Ds0uPlH7NAcUejcOAe_RWXiEurDFLqMwojy5NKg57iLGmW9qDIyW_R1tT67rK-vztGgc14oO6dY_NL2TfXK6z67t3f3cfXt_PP16UW9vvpyebpa15ZjOdcdNd4b01KDBeW0Y9z4EgE4MdJR5g2RxFvqpG6opRikNQ0mrCHctkxySY-rj3vfKcWfG5dnNYRsXd_r0cVNViAYl6JtxXNQ3AootHgaZSAa0kIDBf3wAL2NmzSWzAq4KLtSwPQPdaN7p8Lo45y0XUzVijJGWyJwU6iT_1DldG4INo7OhzL_RwB7gU0x5-S8mlIYdNoqwGppito3RZWmqKUpalc07-8W3pjBdfeK39UoANkDuXyNNy79lehR11_dEsU-</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Nimptsch, Ariane</creator><creator>Fuchs, Beate</creator><creator>Süß, Rosmarie</creator><creator>Zschörnig, Kristin</creator><creator>Jakop, Ulrike</creator><creator>Göritz, Frank</creator><creator>Schiller, Jürgen</creator><creator>Müller, Karin</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7QH</scope><scope>7UA</scope></search><sort><creationdate>20130801</creationdate><title>A simple method to identify ether lipids in spermatozoa samples by MALDI-TOF mass spectrometry</title><author>Nimptsch, Ariane ; Fuchs, Beate ; Süß, Rosmarie ; Zschörnig, Kristin ; Jakop, Ulrike ; Göritz, Frank ; Schiller, Jürgen ; Müller, Karin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c608t-d3bffbb93b07363d46bf650162b8e34fb282fc3e8a53c3018cb5024526c948683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acids</topic><topic>Aldehydes</topic><topic>Alzheimer's disease</topic><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Bioanalysis</topic><topic>Biochemistry</topic><topic>Cats</topic><topic>Cattle</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Deer</topic><topic>Desorption</topic><topic>Digestion</topic><topic>Domestic animals</topic><topic>Electrochemistry</topic><topic>Ether lipids</topic><topic>Ethers</topic><topic>Food Science</topic><topic>Ionization</topic><topic>Laboratory Medicine</topic><topic>Lipids</topic><topic>Male</topic><topic>Mass spectra</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Methods</topic><topic>Monitoring/Environmental Analysis</topic><topic>Physiology</topic><topic>Plasmalogens - analysis</topic><topic>Plasmalogens - chemistry</topic><topic>Properties</topic><topic>Research Paper</topic><topic>Scientific imaging</topic><topic>Species Specificity</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Spectroscopy</topic><topic>Spermatozoa</topic><topic>Spermatozoa - chemistry</topic><topic>Sus scrofa</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Time-of-flight mass spectrometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nimptsch, Ariane</creatorcontrib><creatorcontrib>Fuchs, Beate</creatorcontrib><creatorcontrib>Süß, Rosmarie</creatorcontrib><creatorcontrib>Zschörnig, Kristin</creatorcontrib><creatorcontrib>Jakop, Ulrike</creatorcontrib><creatorcontrib>Göritz, Frank</creatorcontrib><creatorcontrib>Schiller, Jürgen</creatorcontrib><creatorcontrib>Müller, Karin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nimptsch, Ariane</au><au>Fuchs, Beate</au><au>Süß, Rosmarie</au><au>Zschörnig, Kristin</au><au>Jakop, Ulrike</au><au>Göritz, Frank</au><au>Schiller, Jürgen</au><au>Müller, Karin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple method to identify ether lipids in spermatozoa samples by MALDI-TOF mass spectrometry</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>405</volume><issue>21</issue><spage>6675</spage><epage>6682</epage><pages>6675-6682</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Plasmalogens (alkenylacyl glycerophospholipids) are important lipid constituents of many tissues and cells (e.g., selected spermatozoa). Since the molecular weights of plasmalogens overlap with that of diacyl- or alkyl acyl lipids, sophisticated mass spectrometry (MS; including MS/MS) analysis is normally used for the unequivocal identification of plasmalogens. We will show here that a simple matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (without MS/MS capability) in combination with acidic hydrolysis and subsequent derivatization with 2,4-dinitrophenylhydrazine (DNPH) and/or digestion with phospholipase A
2
(PLA
2
) is sufficient to determine the contributions of ether lipids in spermatozoa extracts. As neither diacyl nor alkylacyl lipids are sensitive to acids and do not react with DNPH, the comparison of the mass spectra before and after treatment with acids and/or DNPH addition readily provides unequivocal information about the plasmalogen content. Additionally, the released aldehydes are readily converted into the 2,4-dinitrophenylhydrazones and can be easily identified in the corresponding negative ion mass spectra. Finally, PLA
2
digestion is very useful in confirming the presence of plasmalogens. The suggested method was validated by analyzing roe deer, bovine, boar, and domestic cat spermatozoa extracts and comparing the results with isolated phospholipids.
Figure
ᅟ</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>23812881</pmid><doi>10.1007/s00216-013-7147-z</doi><tpages>8</tpages></addata></record> |
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| source | Springer Nature |
| subjects | Acids Aldehydes Alzheimer's disease Analytical Chemistry Animals Bioanalysis Biochemistry Cats Cattle Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Deer Desorption Digestion Domestic animals Electrochemistry Ether lipids Ethers Food Science Ionization Laboratory Medicine Lipids Male Mass spectra Mass spectrometry Mass spectroscopy Methods Monitoring/Environmental Analysis Physiology Plasmalogens - analysis Plasmalogens - chemistry Properties Research Paper Scientific imaging Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectroscopy Spermatozoa Spermatozoa - chemistry Sus scrofa Tandem Mass Spectrometry - methods Time-of-flight mass spectrometry |
| title | A simple method to identify ether lipids in spermatozoa samples by MALDI-TOF mass spectrometry |
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