The presence of high-risk human papillomavirus (HPV) E6/E7 mRNA transcripts in a subset of sinonasal carcinomas is evidence of involvement of HPV in its etiopathogenesis

• Published in: Virchows Archiv : an international journal of pathology 2015-10, Vol.467 (4), p.405-415
• Main Authors: Laco, Jan, Sieglová, Kateřina, Vošmiková, Hana, Dundr, Pavel, Němejcová, Kristýna, Michálek, Jaroslav, Čelakovský, Petr, Chrobok, Viktor, Mottl, Radovan, Mottlová, Alena, Tuček, Luboš, Slezák, Radovan, Chmelařová, Marcela, Sirák, Igor, Vošmik, Milan, Ryška, Aleš
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Carcinoma, Squamous Cell - mortality; Carcinoma, Squamous Cell - pathology; Carcinoma, Squamous Cell - virology; Cyclin-Dependent Kinase Inhibitor p16; DNA, Viral - analysis; Female; Head and Neck Neoplasms - mortality; Head and Neck Neoplasms - pathology; Head and Neck Neoplasms - virology; Human papillomavirus; Human papillomavirus 16; Human papillomavirus 18; Humans; Immunohistochemistry; Male; Medicine; Medicine & Public Health; Middle Aged; Neoplasm Proteins - analysis; Oncogene Proteins, Viral - genetics; Original Article; Papillomaviridae - isolation & purification; Papillomavirus E7 Proteins - genetics; Paranasal Sinus Neoplasms - mortality; Paranasal Sinus Neoplasms - pathology; Paranasal Sinus Neoplasms - virology; Pathology; Repressor Proteins - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Squamous Cell Carcinoma of Head and Neck; Transcription, Genetic
• ISSN: 0945-6317; 1432-2307
• DOI: 10.1007/s00428-015-1812-x

The aim of the study was to investigate prevalence of high-risk human papillomavirus (HR-HPV) infection in sinonasal carcinomas by immunohistochemistry, in situ hybridization, and polymerase chain reaction, detecting p16 INK4a protein (p16) expression and presence of both HPV DNA and HPV E6/E7 messenger RNA (mRNA). The study comprised 47 males and 26 females, aged 23–83 years (median 62 years), mostly (67 %) with a squamous cell carcinoma (SCC). Of the tumors, 53 % arose in the nasal cavity, 42 % in the maxillary sinus, and 5 % in the ethmoid complex. The follow-up period ranged 1–241 months (median 19 months). HPV16, HPV18, or HPV35 were detected in 18/73 (25 %) tumors, 17 SCCs, and 1 small cell neuroendocrine carcinoma. There was a strong correlation between results of HPV detection methods and p16 expression ( p