Transcription factors from Sox family regulate expression of zebrafish Gla-rich protein 2 gene

GRP is a vitamin K-dependent protein with orthologs in all vertebrate taxonomic groups and two paralogs in teleosts. However, no data is available about GRP transcriptional gene regulation. We report a functional promoter for zebrafish grp2 gene regulated by Sox9b, Sox10, Ets1 and Mef2ca as determin...

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Bibliographic Details
Published in:Gene 2015-11, Vol.572 (1), p.57-62
Main Authors: Fazenda, C., Conceição, N., Cancela, M.L.
Format: Article
Language:English
Subjects:
Animals
Danio rerio
Freshwater
Gene Expression Regulation
Gene regulation
Gla-rich protein
Humans
In Vitro Techniques
MEF2 Transcription Factors - genetics
MEF2 Transcription Factors - metabolism
Oryzias - genetics
Oryzias - metabolism
Promoter Regions, Genetic
Proteins - genetics
Proto-Oncogene Proteins c-ets - genetics
Proto-Oncogene Proteins c-ets - metabolism
Regulatory Elements, Transcriptional
Sox family
SOX Transcription Factors - genetics
SOX Transcription Factors - metabolism
SOX9 Transcription Factor - genetics
SOX9 Transcription Factor - metabolism
SOXE Transcription Factors - genetics
SOXE Transcription Factors - metabolism
Species Specificity
Teleostei
Transcription factors
Transcriptional Activation
Zebrafish - genetics
Zebrafish - metabolism
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
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Description
Summary:GRP is a vitamin K-dependent protein with orthologs in all vertebrate taxonomic groups and two paralogs in teleosts. However, no data is available about GRP transcriptional gene regulation. We report a functional promoter for zebrafish grp2 gene regulated by Sox9b, Sox10, Ets1 and Mef2ca as determined by in vitro assays. This was confirmed in vivo for Sox9b and Sox10. Due to the high conservation between human GRP and grp2, its zebrafish ortholog, our results are relevant for the study of human GRP gene regulation and provide new insights towards understanding GRP function. •We report the identification of a functional promoter for zebrafish grp2 gene.•Transcription factors Sox, Mef2 and Ets1 are regulators for grp2 gene.•Results confirmed that Sox9b and Sox10 were able to regulate grp2 promoter in vivo.•Sox10 is a bona fide transcription regulator of grp2 gene.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2015.06.079