Magnetic actuator for the control and mixing of magnetic bead-based reactions on-chip

While magnetic bead (MB)-based bioassays have been implemented in integrated devices, their handling on - chip is normally either not optimal—i.e. only trapping is achieved, with aggregation of the beads—or requires complex actuator systems. Herein, we describe a simple and low-cost magnetic actuato...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2014-10, Vol.406 (26), p.6607-6616
Main Authors: Berenguel-Alonso, Miguel, Granados, Xavier, Faraudo, Jordi, Alonso-Chamarro, Julián, Puyol, Mar
Format: Article
Language:English
Subjects:
Actuators
Agglomeration
Analytical Chemistry
Beads
Bioassays
Biochemistry
Biological analysis
Biological assay
Biosensors
Cadmium
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Devices
E coli
Enzymes
Equipment Design
Escherichia coli Infections - microbiology
Escherichia coli O157 - isolation & purification
Fluorescence
Food Science
Humans
Immunoassay
Immunomagnetic Separation - economics
Immunomagnetic Separation - instrumentation
Laboratory Medicine
Limit of Detection
Magnetic Phenomena
Microfluidic Analytical Techniques - economics
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Monitoring/Environmental Analysis
Nucleic acids
Reagents
Research Paper
Trapping
Water analysis
Water sampling
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Description
Summary:While magnetic bead (MB)-based bioassays have been implemented in integrated devices, their handling on - chip is normally either not optimal—i.e. only trapping is achieved, with aggregation of the beads—or requires complex actuator systems. Herein, we describe a simple and low-cost magnetic actuator to trap and move MBs within a microfluidic chamber in order to enhance the mixing of a MB-based reaction. The magnetic actuator consists of a CD-shaped plastic unit with an arrangement of embedded magnets which, when rotating, generate the mixing. The magnetic actuator has been used to enhance the amplification reaction of an enzyme-linked fluorescence immunoassay to detect Escherichia coli O157:H7 whole cells, an enterohemorrhagic strain, which have caused several outbreaks in food and water samples. A 2.7-fold sensitivity enhancement was attained with a detection limit of 603 colony-forming units (CFU) /mL, when employing the magnetic actuator. Graphical Abstract ᅟ
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-014-8100-5