Optimal attenuation of a PR8-derived mouse pathogenic H5N1 recombinant virus for testing antigenicity and protective efficacy in mice

Highlights • A mouse-pathogenic H5N1 LPAIV without adaptation was identified. • The PR8-derived H5N1 recombinant virus was still mouse-pathogenic. • Replacement of PR8 NS or PB2 with non-pathogenic (np) NS or PB2 eradicated the pathogenicity. • The np NS may be useful for generating nonpathogenic an...

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Bibliographic Details
Published in:Vaccine 2015-11, Vol.33 (46), p.6314-6319
Main Authors: Kim, Il-Hwan, Kwon, Hyuk-Joon, Park, Jae-Keun, Song, Chang-Seon, Kim, Jae-Hong
Format: Article
Language:English
Subjects:
Allergy and Immunology
Amino acids
Animals
Antigenicity
Antigens, Viral - genetics
Antigens, Viral - immunology
Avian flu
Avian influenza virus
Body Weight
Disease Models, Animal
Efficiency
Epidemics
Female
Genes
Genetics
H5N1
Immunization
Influenza A Virus, H5N1 Subtype - genetics
Influenza A Virus, H5N1 Subtype - immunology
Influenza A Virus, H5N1 Subtype - pathogenicity
Influenza A Virus, H9N2 Subtype - genetics
Influenza A Virus, H9N2 Subtype - immunology
Influenza A Virus, H9N2 Subtype - pathogenicity
Lung - virology
Mice, Inbred BALB C
Orthomyxoviridae
Orthomyxoviridae Infections - immunology
Orthomyxoviridae Infections - pathology
Orthomyxoviridae Infections - prevention & control
Orthomyxoviridae Infections - virology
Pathogenicity
Pathogens
Plasmids
PR8-derived recombinant virus
Protective efficacy
Recombination, Genetic
Reverse Genetics - methods
Survival Analysis
Virulence
Viruses
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Description
Summary:Highlights • A mouse-pathogenic H5N1 LPAIV without adaptation was identified. • The PR8-derived H5N1 recombinant virus was still mouse-pathogenic. • Replacement of PR8 NS or PB2 with non-pathogenic (np) NS or PB2 eradicated the pathogenicity. • The np NS may be useful for generating nonpathogenic and immunogenic PR8-derived recombinant virus. • The np NS or PB2-bearing H5N1 viruses were obviously protective against H5N1 HPAIV.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2015.09.048