Development and validation of a specific and sensitive LC-MS/MS method for quantification of urinary catecholamines and application in biological variation studies

Catecholamines are a class of biogenic amines that play an important role as neurotransmitters and hormones. We developed and validated a rapid, specific and sensitive LC-MS/MS method for quantitative determination of catecholamines in human urine. Linearity, specificity, sensitivity, precision, acc...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2014-11, Vol.406 (28), p.7287-7297
Main Authors: Li, Xiaoguang (Sunny), Li, Shu, Wynveen, Paul, Mork, Kathy, Kellermann, Gottfried
Format: Article
Language:English
Subjects:
Amines
Analytical Chemistry
Biochemistry
Biogenic amines
Biological
Catecholamine
Catecholamines
Catecholamines - urine
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chromatography, Liquid - methods
Circadian Rhythm
Diurnal variations
Dopamine
Epinephrine
Excretion
Food Science
Hormones
Human
Humans
Laboratory Medicine
Linearity
Living conditions
Metabolites
Methods
Monitoring/Environmental Analysis
Morning
Neurotransmitters
Norepinephrine
Reference Values
Research Paper
Sensitivity analysis
Sensitivity and Specificity
Solid Phase Extraction
Solid phases
Stability analysis
Tandem Mass Spectrometry - methods
Urine
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Summary:Catecholamines are a class of biogenic amines that play an important role as neurotransmitters and hormones. We developed and validated a rapid, specific and sensitive LC-MS/MS method for quantitative determination of catecholamines in human urine. Linearity, specificity, sensitivity, precision, accuracy, matrix effect, carryover, analyte stability, method comparison and reference range were evaluated. The catecholamine measurements were not affected by 35 structurally-related drugs and metabolites. The outstanding specificity was achieved by use of a specific diphenylborate-based solid phase extraction and subsequent selective LC-MS/MS analysis. Excellent sensitivity, accuracy and precision (average intra-assay variations
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-014-8120-1