Sample preparation strategies for improving the identification of membrane proteins by mass spectrometry

Despite enormous advances in the mass spectrometry and proteomics fields during the last two decades, the analysis of membrane proteins still remains a challenge for the proteomic community. Membrane proteins play a wide number of key roles in several cellular events, making them relevant target mol...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2015-07, Vol.407 (17), p.4893-4905
Main Authors: Alfonso-Garrido, Javier, Garcia-Calvo, Estefania, Luque-Garcia, Jose L
Format: Article
Language:English
Subjects:
Analytical Chemistry
Animals
Biochemistry
cell communication
Cellular
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chromatography
Chromatography, Liquid - methods
Detergents - chemistry
Efficiency
Enzymes
Filtration - methods
Food Science
Humans
Hydrophobic and Hydrophilic Interactions
Identification
Laboratory Medicine
Mass spectrometry
Mass Spectrometry - methods
Membrane proteins
Membrane Proteins - analysis
Membrane Proteins - isolation & purification
Membranes
Models, Molecular
monitoring
Monitoring/Environmental Analysis
Nanoparticles - chemistry
Peptides
Proteins
Proteomics
Proteomics - methods
Review
Sample preparation
Scientific imaging
Solubility
Solvents
Solvents - chemistry
Strategy
Surfactants
Surveillance
Tasks
Vaccines
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Summary:Despite enormous advances in the mass spectrometry and proteomics fields during the last two decades, the analysis of membrane proteins still remains a challenge for the proteomic community. Membrane proteins play a wide number of key roles in several cellular events, making them relevant target molecules to study in a significant variety of investigations (e.g., cellular signaling, immune surveillance, drug targets, vaccine candidates, etc.). Here, we critically review the several attempts that have been carried out on the different steps of the sample preparation procedure to improve and modify existing conventional proteomic strategies in order to make them suitable for the study of membrane proteins. We also revise novel techniques that have been designed to tackle the difficult but relevant task of identifying and characterizing membrane proteins.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-015-8732-0