DNA methylation of genes of the main components of the telomerase complex in Danio rerio

The methylation status of the genes of telomerase reverse transcriptase ( tert ) and telomerase RNA ( terc ) was determined in brain tissues of Danio rerio of different age. It is found that, regardless of the age of fish, the regulatory region of the tert gene was completely methylated, whereas the...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2015-09, Vol.464 (1), p.329-332
Main Authors: Belova, E. V., Kozlov, A. E., Shubernetskaya, O. S., Zvereva, M. I., Shpanchenko, O. V., Dontsova, O. A.
Format: Article
Language:English
Subjects:
Aging - genetics
Aging - metabolism
Animals
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biophysics and Molecular Biology
Brain - growth & development
Brain - metabolism
CpG Islands
Danio rerio
DNA Methylation
Genes
Life Sciences
Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA - genetics
Telomerase
Telomerase - genetics
Zebrafish - genetics
Zebrafish - metabolism
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Summary:The methylation status of the genes of telomerase reverse transcriptase ( tert ) and telomerase RNA ( terc ) was determined in brain tissues of Danio rerio of different age. It is found that, regardless of the age of fish, the regulatory region of the tert gene was completely methylated, whereas the coding region remained unmethylated in all cases. The level of methylation of the region located downstream of the coding region of the terc gene changes with age. This region was analyzed in the samples of other tissues, and its methylation status was also nonuniform. The alteration of the methylation status in the 3'-untranslated region of the terc gene suggests the possibility of transcription of the antisense strand in this region.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672915050166