Controllable Drug Release System in Living Cells Triggered by Enzyme–Substrate Recognition

• Published in: ACS applied materials & interfaces 2015-12, Vol.7 (48), p.26811-26818
• Main Authors: Liu, Pengchang, Wang, Xiaoliang, Hiltunen, Kalervo, Chen, Zhijun
• Format: Article
• Language: English
• Subjects: Delayed-Action Preparations; Drug Delivery Systems; Drug Liberation; Glutathione - metabolism; Glutathione Transferase - metabolism; Microbial Viability; Microscopy, Confocal; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Porosity; Rhodamines - chemistry; Saccharomyces cerevisiae - cytology; Silicon Dioxide - chemistry; Substrate Specificity
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.5b08914

Vehicles can deliver the drug molecules into cells, yet immunoreaction of the commonly used capping agents and release triggers limit their biomedical use. This shortcoming might be circumvented through replacing these chemicals with certain biomolecules. Here, we show a new and facile way to encapsulate the drug delivery vehicles and release the cargos in a highly controllable manner via modulating supramolecular interactions between enzyme, substrate, and vehicle. The cargo release from the vehicles within cells can be achieved upon substrate treatment. Yeast cells were used, allowing for a fast and cost-effective way for imaging and morphological analysis. We believe this new platform can be readily extended to various carrier systems for different purposes based on shifting the recognition pattern of enzyme–substrate pairs.