Separation and identification of Aconitum alkaloids and their metabolites in human urine

To study the safety of Aconitum medicinal herbs in clinic and identify Aconitum alkaloids poisoning in forensic medicine, Aconitum alkaloids and their metabolites were separated and identified in human urine by liquid chromatography-electrospray ionization-multi-stage mass spectrometry (LC-ESI-MS n)...

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Bibliographic Details
Published in:Toxicon (Oxford) 2005-10, Vol.46 (5), p.500-506
Main Authors: Zhang, Hong-Gui, Sun, Ying, Duan, Ming-Yu, Chen, Yu-Juan, Zhong, Da-Fang, Zhang, Han-Qi
Format: Article
Language:English
Subjects:
16- O-demethylaconitine
16- O-demethylhypaconitine
Aconitine poisoning
Aconitum
Aconitum - chemistry
Aconitum alkaloids
Alkaloids - isolation & purification
Alkaloids - urine
Biological and medical sciences
Biotransformation
Chromatography, High Pressure Liquid
Drugs, Chinese Herbal - metabolism
Female
Humans
Liquid chromatography-mass spectrometry
Medical sciences
metabolites
Plant poisons toxicology
Spectrometry, Mass, Electrospray Ionization
Toxicology
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Summary:To study the safety of Aconitum medicinal herbs in clinic and identify Aconitum alkaloids poisoning in forensic medicine, Aconitum alkaloids and their metabolites were separated and identified in human urine by liquid chromatography-electrospray ionization-multi-stage mass spectrometry (LC-ESI-MS n) and chemical pathway of metabolism was investigated. The alkaloids and their metabolites in the urine sample were extracted with solid-phase cartridges and separated by HPLC with acetonitrile-water-formic acid (40:60:0.5) mobile phase. Structures of five metabolites and three parent Aconitum alkaloids were identified with multi-stage mass spectrometry data through comparison with authentic substances as aconitine (M 1), mesaconitine (M 2), hypaconitine (M 3), benzoylaconine (M 4), benzoylmesaconine (M 5), benzoylhypaconine (M 6), 16- O-demethylaconitine (M 7) and 16- O-demethylhypaconitine (M 8), respectively. Among them, M 8 was identified and reported for the first time. Metabolic pathways of Aconitum alkaloids in human body were proposed.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2005.06.014