Oxidative mutagenesis in Escherichia coli strains lacking ROS-scavenging enzymes and/or 8-oxoguanine defenses

• Published in: Environmental and molecular mutagenesis 2000, Vol.35 (1), p.22-30
• Main Authors: Ruiz-Laguna, Julia, Prieto-Álamo, María-José, Pueyo, Carmen
• Format: Article
• Language: English
• Subjects: 8-Oxoguanine; Bacteriology; Biological and medical sciences; catalase; DNA Repair; Escherichia coli; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - metabolism; Fpg; fpg gene; Free Radical Scavengers; Fundamental and applied biological sciences. Psychology; Genetics; Guanine - analogs & derivatives; Guanine - metabolism; katE gene; katG gene; Microbiology; Mutagenesis; MutY; mutY gene; MutY protein; Oxidative Stress; Reactive Oxygen Species; SOD; sodA gene; sodB gene; SOS Response (Genetics); spontaneous mutations; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism
• ISSN: 0893-6692; 1098-2280
• DOI: 10.1002/(SICI)1098-2280(2000)35:1<22::AID-EM4>3.0.CO;2-X

Escherichia coli strains with different combinations of null mutations in the katG, katE, sodA, sodB, fpg, and mutY genes were constructed to compare their spontaneous mutation frequencies and sensitivities to various oxidants with those of bacteria solely deficient in catalase (katG katE) or cytosolic superoxide dismutase (sodA sodB) and the parental strain possessing a full complement of these enzymes. The MutY DNA glycosylase represented the major protection against the mutagenic consequences of processes associated with normal aerobic metabolism. Spontaneous mutagenesis in MutY‐lacking bacteria was not influenced by the absence of (A)BC excinuclease or the presence of MucAB proteins, a result consistent with 8‐oxoguanine being a principal premutational lesion. In contrast, catalase and SOD represented the major protection against the genotoxic consequences of bursts of oxidative stress caused by reactive‐oxygen‐generating compounds. Therefore, only bacteria simultaneously defective in both katG and katE or sodA and sodB genes were hypersensitive with respect to mutability by peroxide and superoxide, respectively. These data suggest that oxidative lesions other than 8‐oxoguanine contribute to mutagenesis by hydrogen peroxide and redox‐cycling chemicals. Environ. Mol. Mutagen. 35:22–30, 2000 © 2000 Wiley‐Liss, Inc.