Replicon Cell Culture System as a Valuable Tool in Antiviral Drug Discovery against Hepatitis C Virus

Discovery of potential therapeutics against hepatitis C virus (HCV) infection has been hampered in the past decade by the inability to grow this virus in tissue culture and by the lack of robust small animal models. This situation has been improved by the recent development of a selectable HCV repli...

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Bibliographic Details
Published in:Antiviral Chemistry and Chemotherapy 2005-02, Vol.16 (1), p.1-12
Main Authors: Horscroft, Nigel, Lai, Vicky CH, Cheney, Wayne, Yao, Nanhua, Wu, Jim Z, Hong, Zhi, Zhong, Weidong
Format: Article
Language:English
Subjects:
Animal models
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiviral activity
Antiviral agents
Antiviral Agents - pharmacology
Biological and medical sciences
Cell culture
Drug discovery
Enzyme Inhibitors - pharmacology
Hepacivirus - drug effects
Hepacivirus - enzymology
Hepacivirus - physiology
Hepatitis
Hepatitis C
Hepatitis C - drug therapy
Hepatitis C - virology
Hepatitis C virus
Humans
Interferon
Medical sciences
Pharmacology. Drug treatments
Replicon - physiology
Ribonucleic acid
RNA
RNA Replicase - antagonists & inhibitors
Tissue culture
Tumor Cells, Cultured
Virus Cultivation - methods
Virus Replication - drug effects
Virus Replication - physiology
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Summary:Discovery of potential therapeutics against hepatitis C virus (HCV) infection has been hampered in the past decade by the inability to grow this virus in tissue culture and by the lack of robust small animal models. This situation has been improved by the recent development of a selectable HCV replicon cell culture system. For the first time, drug discovery scientists are able to screen large compound collections using the replicon cell culture system to identify small molecules with the potential to inhibit HCV RNA replication. The replicon system has also been used to elucidate inhibitors' antiviral mechanism of action and to optimize antiviral potency. In this review, we will summarize the recent development of HCV replicon cell culture system and its use in anti-HCV drug discovery. The antiviral activities of promising lead compounds are also reviewed.
ISSN:2040-2066
0956-3202
2040-2066
DOI:10.1177/095632020501600101