Isolation and characterization of microsatellite markers in Phytophthora ramorum, the causal agent of sudden oak death

We describe specific primers and conditions to amplify two dinucleotide and five trinucleotide microsatellite DNA loci isolated from the oomycete Phytophthora ramorum, the causal agent of sudden oak death. The primer sets were tested on 14–30 isolates from North America and Europe. Seven of 14 loci...

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Bibliographic Details
Published in:Molecular ecology notes 2004-12, Vol.4 (4), p.672-674
Main Authors: Prospero, S, Black, J.A, Winton, L.M
Format: Article
Language:English
Subjects:
alleles
dinucleotide repeats
DNA primers
forest pathology
fungal diseases of plants
genetic markers
loci
microsatellite repeats
molecular sequence data
nucleotide sequences
Oomycetes
Phytophthora
Phytophthora ramorum
plant disease
plant pathogenic fungi
polymerase chain reaction
Quercus
tree diseases
trinucleotide repeats
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Summary:We describe specific primers and conditions to amplify two dinucleotide and five trinucleotide microsatellite DNA loci isolated from the oomycete Phytophthora ramorum, the causal agent of sudden oak death. The primer sets were tested on 14–30 isolates from North America and Europe. Seven of 14 loci differentiated between A1 and A2 mating types. All seven loci successfully amplified DNA isolated from infected plant tissue. Four loci may be useful for the diagnosis of P. ramorum because they do not amplify closely related Phytophthora species.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2004.00778.x