Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains of Drosophila melanogaster

The Rst(2)DDT locus (loci) in Drosophila is associated with the over-expression of two cytochrome P450 genes, Cyp6g1 and Cyp12d1. Using northern and western blot analysis we observed the expression pattern of these two genes in two DDT susceptible (Canton-S and 91-C) and three DDT resistant strains...

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Bibliographic Details
Published in:Insect molecular biology 2005, Vol.14 (1), p.69-77
Main Authors: Festucci-Buselli, R.A, Carvalho-Dias, A.S, Oliveira-Andrade, M. de, Caixeta-Nunes, C, Li, H.M, Stuart, J.J, Muir, W, Scharf, M.E, Pittendrigh, B.R
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Blotting, Northern
Blotting, Western
cytochrome P-450
Cytochrome P-450 Enzyme System - biosynthesis
Cytochrome P-450 Enzyme System - genetics
DDT
DDT (pesticide)
developmental stages
Drosophila melanogaster
Drosophila melanogaster - enzymology
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Drosophila Proteins - biosynthesis
Drosophila Proteins - genetics
Enzyme Induction - drug effects
Female
gene expression
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
gene induction
gene overexpression
genetic resistance
insecticide
insecticide resistance
Insecticide Resistance - physiology
Insecticides
Male
messenger RNA
metabolic
metabolic detoxification
Molecular Sequence Data
pesticide
resistance
resistance mechanisms
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
strain differences
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Summary:The Rst(2)DDT locus (loci) in Drosophila is associated with the over-expression of two cytochrome P450 genes, Cyp6g1 and Cyp12d1. Using northern and western blot analysis we observed the expression pattern of these two genes in two DDT susceptible (Canton-S and 91-C) and three DDT resistant strains (Wisconsin, 91-R and Hikone-R). In Canton-S and 91-R, the CYP6G1 protein was constitutively expressed throughout development. In the Wisconsin strain, CYP6G1 was not expressed in third instar larvae unless the larvae are exposed to DDT. CYP12D1 protein was only expressed in adults. Cyp12d1 mRNA is induced in DDT resistant strains post-exposure to DDT and the expression patterns of Cyp12d1 mRNA varied across DDT resistant strains. Our data support the hypothesis that there is evolutionary plasticity in the expression patterns of P450s associated with metabolic pesticide resistance.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.2005.00532.x