A high density microelectrode array biosensor for detection of E. coli O157:H7

A high density microelectrode array biosensor was developed for the detection of Escherichia coli O157:H7. The biosensor was fabricated from (1 0 0) silicon with a 2 μm layer of thermal oxide as an insulating layer, an active area of 9.6 mm 2 and consists of an interdigitated gold electrode array. T...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2005-02, Vol.20 (8), p.1662-1667
Main Authors: Radke, Stephen M., Alocilja, Evangelyn C.
Format: Article
Language:English
Subjects:
Antibodies - analysis
Antibodies - immunology
Antibody
Bacteria
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Electric Impedance
Electrochemistry - instrumentation
Electrochemistry - methods
Electrode
Equipment Design
Equipment Failure Analysis
Escherichia coli
Escherichia coli O157 - immunology
Escherichia coli O157 - isolation & purification
Food Analysis - instrumentation
Food Analysis - methods
Food Contamination - analysis
Food Microbiology
Fundamental and applied biological sciences. Psychology
Immunoassay - instrumentation
Immunoassay - methods
Methods. Procedures. Technologies
Microelectrodes
Microfabrication
Miniaturization
Pathogen
Reproducibility of Results
Sensitivity and Specificity
Various methods and equipments
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Summary:A high density microelectrode array biosensor was developed for the detection of Escherichia coli O157:H7. The biosensor was fabricated from (1 0 0) silicon with a 2 μm layer of thermal oxide as an insulating layer, an active area of 9.6 mm 2 and consists of an interdigitated gold electrode array. The sensor surface was functionalised for bacterial detection using heterobifunctional crosslinkers and immobilised polyclonal antibodies to create a biological sensing surface. Bacteria suspended in solution became attached to the immobilised antibodies when the biosensor was tested in liquid samples. The change in impedance caused by the bacteria was measured over a frequency range of 100 Hz–10 MHz. The biosensor was evaluated for E. coli O157:H7 detection in pure culture and inoculated food samples. The biosensor was able to discriminate between cellular concentrations of 10 4–10 7 CFU/mL and has applications in detecting pathogens in food samples.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2004.07.021