Evaluation of the SOS/ umu-test post-treatment assay for the detection of genotoxic activities of pure compounds and complex environmental mixtures

This study presents an evaluation of the SOS/ umu-test after introducing an additional dilution and incubation in the post-treatment assay. This treatment reduces the influence of coloured test compounds that otherwise affect the colorimetric determination of the β-galactosidase activity and the bac...

Full description

Saved in:
Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2000-03, Vol.466 (2), p.161-171
Main Authors: Hamer, Bojan, Bihari, Nevenka, Reifferscheid, Georg, Zahn, Rudolf K, Müller, W.E.G, Batel, Renato
Format: Article
Language:English
Subjects:
4-nitroquinoline 1-oxide
4-nitroquinoline-N-oxide
b-Galactosidase
Biological and medical sciences
Chemical mutagenesis
Environmental pollution
Filamentous bacteria
Genotoxicity assay
genotoxins
Medical sciences
N-Methyl-N'-nitro-N-nitrosoguanidine
Post-treatment assay
Salmonella typhimurium
SOS response
SOS/ umu-test
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study presents an evaluation of the SOS/ umu-test after introducing an additional dilution and incubation in the post-treatment assay. This treatment reduces the influence of coloured test compounds that otherwise affect the colorimetric determination of the β-galactosidase activity and the bacterial growth measurement during the testing of complex environmental samples. The post-treatment assay significantly increased the β-galactosidase activity and consequently the enzyme induction ratios at higher doses of model genotoxins 4-nitroquinoline- N-oxide, N-methyl- N′-nitro- N-nitrosoguanidine, 2-aminoanthracene, benzo( a)pyrene with low or no effect on the sensitivity of the test itself. On the other hand tests of environmental extracts indicated significant increases in sensitivity after additional incubation. 4-Nitroquinoline- N-oxide treatments of bacteria in the test affected cell division and caused filamentous growth. The size of filamentous bacteria and incidence rate of the length categories was positively correlated with the concentrations of genotoxins. Presence of filamentous tester bacteria proved induction of SOS response and genotoxic activity of environment samples in SOS/ umu-test.
ISSN:1383-5718
1879-3592
DOI:10.1016/S1383-5718(00)00016-4