Insights into the physiological responses of the facultative halophyte Aeluropus littoralis to the combined effects of salinity and phosphorus availability

• Published in: Journal of plant physiology 2015-09, Vol.189, p.1-10
• Main Authors: Talbi Zribi, Ons, Barhoumi, Zouhaier, Kouas, Saber, Ghandour, Mohamed, Slama, Ines, Abdelly, Chedly
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Aeluropus littoralis; Chlorides - metabolism; Excretion; Osmosis; Phosphorus - deficiency; Plant Leaves - drug effects; Plant Leaves - physiology; Plant Roots - drug effects; Plant Roots - physiology; Plant Shoots - drug effects; Plant Shoots - physiology; Poaceae - drug effects; Poaceae - physiology; Potassium - metabolism; Proline - metabolism; Salinity; Salt-Tolerance - drug effects; Salt-Tolerant Plants; Sodium - metabolism; Sodium Chloride - pharmacology; Stress, Physiological; Water - physiology
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2015.08.007

In this work, we investigate the physiological responses to P deficiency (5μM KH2PO4=D), salt stress (400mM NaCl=C+S), and their combination (D+S) on the facultative halophyte Aeluropus littoralis to understand how plants adapt to these combined stresses. When individually applied, both P deficiency and salinity significantly restricted whole plant growth, with a more marked effect of the latter stress. However, the effects of the two stresses were not additive in plant biomass production since the response of plants to combined salinity and P deficiency was similar to that of plants grown under salt stress alone. In addition the observed features under salinity alone are kept when plants are simultaneously subjected to the combined effects of salinity and P deficiency such as biomass partitioning; the synthesis of proline and the K+/Na+ selectivity ratio. Thus, increasing P availability under saline conditions has no significant effect on salt tolerance in this species. Plants cultivated under the combined effects of salinity and P deficiency exhibited the lowest leaf water potential. This trend was associated with a high accumulation of Na+, Cl− and proline in shoots of salt treated plants suggesting the involvement of these solutes in osmotic adjustment. Proline could be involved in other physiological processes such as free radical scavenging. Furthermore, salinity has no significant effect on phosphorus acquisition when combined with a low P supply and it significantly decreased this parameter when combined with a sufficient P supply. This fact was probably due to salt’s effect on P transporters. In addition, shoot soluble sugars accumulation under both P deficiency treatments with and without salt likely play an important role in the adaptation of A. littoralis plants to P shortage applied alone or combined with salinity. Moreover, there was a strong correlation between shoot and root intracellular acid phosphatase activity and phosphorus use efficiency which strengthens the assumption that intracellular acid phosphatase enzymes are involved in P remobilization in this species. Finally, our results showed that P availability has no significant effect on salt excretion in A. littorlais which suggests that independently of the P status in the plant, excretion remains priority over other functions requiring energy such as growth. This result could also indicate that salt excretion is not energy-dependent in this species.