Effects of different antibacterial agents on enamel in a biofilm caries model

• Published in: Journal of Oral Science 2015, Vol.57(4), pp.367-372
• Main Authors: Savas, Selcuk, Kucukyılmaz, Ebru, Celik, Esra U., Ates, Mustafa
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Biofilms; dental caries; Dental Caries - microbiology; Dental Enamel - drug effects; Dentistry; fluoride; Humans; Models, Biological; oral biofilm; Streptococcus mutans; Streptococcus mutans - drug effects; Streptococcus mutans - growth & development; Streptococcus mutans - isolation & purification
• ISSN: 1343-4934; 1880-4926
• DOI: 10.2334/josnusd.57.367

Using a mature biofilm model, the aim of this study was to evaluate the effectiveness of different antibacterial agents in comparison with silver diamine fluoride (SDF). Forty-eight saliva-coated enamel slabs were inoculated with Streptococcus mutans monospecies biofilm. The biofilms were then exposed to 10% sucrose in tryptone yeast-extract culture medium, 8 times per day for 7 days. After the biofilm growth period, the enamel slabs were treated with one of the following substances: 1) distilled water; 2) SDF; 3) acidulated phosphate fluoride (APF); 4) ammonium hexafluorosilicate (AHF); 5) ammonium hexafluorosilicate + cetylpyridinium chloride (AHF+CPC); or 6) 0.2% chlorhexidine (CHX). After these treatment procedures, the samples were incubated at 37ºC for 2 days, and the numbers of viable microorganisms in the biofilms were counted. The number of viable bacteria was significantly reduced by all of the antibacterial agents (P < 0.05). However, SDF showed the highest antibacterial activity (P < 0.05), and the effectiveness of the other agents was lower (P < 0.05). SDF has a highly effective antibacterial action against cariogenic Streptococcus mutans biofilm; none of the other fluoride agents used in this study, or 0.2 CHX agent, showed an antibacterial effect comparable to that of SDF. (J Oral Sci 57, 367-372, 2015)