Toxicological evaluation of the natural products and some semisynthetic derivatives of Heterotheca inuloides Cass (Asteraceae)

• Published in: Journal of ethnopharmacology 2015-12, Vol.175, p.256-265
• Main Authors: Rodríguez-Chávez, José Luis, Coballase-Urrutia, Elvia, Sicilia-Argumedo, Gloria, Ramírez-Apan, Teresa, Delgado, Guillermo
• Format: Article
• Language: English
• Subjects: Acute toxicity; Animals; Antineoplastic Agents - toxicity; Artemia - drug effects; Asteraceae; Biological Products - toxicity; Cadinanes; Cell Line; Cell Line, Tumor; Cell Survival - drug effects; Cytotoxicity; Female; Flowers; Heterotheca inuloides; Mexican arnica; Mice; RAW264.7 macrophages; Toxicity Tests, Acute
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2015.08.055

Heterotheca ineuloides Cass (Asteraceae), popularly known as árnica mexicana, is widely used in Mexican traditional medicine to treat bruises, dermatological problems, rheumatic pains, and other disorders as cancer. The major constituents in H. inuloides are cadinane type sesquiterpenes, flavonoids and phytosterols. Compounds with a cadinane skeleton have been proved to possess cytotoxic activity against human-tumor cell lines and brine shrimp, and display toxic effects in different animal species. Although this plant has been widely used, there is little available information on the safety and toxicity especially of pure compounds. Evaluate the potential toxicity of the natural products isolated from H. inuloides and some semisynthetic derivatives. The toxic aspects of the following natural products isolated from dried flowers of H. inuloides: 7-hydroxy-3,4-dihydrocadalene (1), 7-hydroxycadalene (2), 3,7-dihydroxy-3(4H)-isocadalen-4-one (3), (1R,4R)-1-hydroxy-4H-1,2,3,4- tetrahydrocadalen-15-oic acid (4), D-chiro-inositol (5), quercetin (6), quercetin-3,7,3′-trimethyl ether (7), quercetin-3,7,3′,4′-tetramethyl ether (8), eriodictyol-7,4′-dimethyl ether (9), α-spinasterol (10), caryolan-1,9β-diol (11) and 7-(3,3-dimethylallyloxy)-coumarin (12) as well as the toxic aspects of the semisynthetic compounds 7-acetoxy-3,4-dihydrocadalene (13), 7-benzoxy-3,4-dihydrocadalene (14), 7-acetoxycadalene (15), 7-benzoxycadalene (16), quercetin pentaacetate (17), 7-hydroxycalamenene (18), 3,8-dimethyl-5-(1-methylethyl)-1,2-naphthoquinone (19), and 4-isopropyl-1,6-dimethylbenzo[c]oxepine-7,9-dione (20). Toxic activities of compounds were determined by sulforhodamine B (SRB) assay, Artemia salina assay, RAW264.7 macrophage cells. Additionally, the acute toxicity in mouse of compound 1, the major natural sesquiterpene isolated from the acetone extract, was evaluated. The best cytotoxicity activity was observed for mansonone C (19) on K562 cell line with IC50 1.45±0.14μM, for 7-hydroxycadalene (2) on HCT-15 cell line with IC50 18.89±1.2μM, and for quercetin pentaacetate (17) on MCF-7 cell line with IC50 22.57±2.4μM. Sesquiterpenes mansonone C (19) and 7-hydroxy-3,4-dihydrocadalene (1) caused the strongest deleterious effects against A. salina with IC50 39.4±1.07, and 45.47±1.74μM, respectively. The number of viable RAW 264.7 cells was reduced with sesquiterpenes 1 and 2 by more than 90%. In addition, the acute study of 1 revealed no lethal effects at 300mg/kg body weight, ho