Murine Bone Marrow-Derived Mast Cells Exhibit Evidence of Both Apoptosis and Oncosis After IL-3 Deprivation

IL-3 deprivation has been reported to induce apoptosis of bone marrow-derived mast cells. In order to evaluate this type of cell death further, we employed trypan blue and propidium iodide stainings, photometric enzyme immunoassay, fluorescence measurement of caspase-3, DNA electrophoresis, flow cyt...

Full description

Saved in:
Bibliographic Details
Published in:Immunological investigations 2000-01, Vol.29 (1), p.51-60
Main Authors: Park, Bong Soo, Kim, Gyoo Cheon, Baek, Soo Jin, Kim, Nam Deuk, Kim, Yang Soon, Kim, Seok Kwon, Jeong, Min Ho, Lim, Young Jin, Yoo, Young Hyun
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
Bone Marrow Cells - ultrastructure
Cell Death
Cells, Cultured
Interleukin-3 - metabolism
Mast Cells - cytology
Mast Cells - metabolism
Mast Cells - ultrastructure
Mice
Mice, Inbred BALB C
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:IL-3 deprivation has been reported to induce apoptosis of bone marrow-derived mast cells. In order to evaluate this type of cell death further, we employed trypan blue and propidium iodide stainings, photometric enzyme immunoassay, fluorescence measurement of caspase-3, DNA electrophoresis, flow cytometry and transmission electron microscopy. In this experiment, although several evidences supporting apoptosis were demonstrated some findings were not consistent with typical apoptosis. On the other hand, electron microscopical observation demonstrated that most cells from all the time phases after IL-3 deprivation showed the morphology of typical oncosis, i.e. cell swelling, disintegration of ultrastructure and subsequent karyolysis. Only a small number of cells from the later time phases showed apoptotic morphology. We here suggest that BMMCs undergo both apoptosis and oncosis after IL-3 deprivation and that the dominant type of prelethal change is oncosis in all time phases, although apoptosis also plays a partial role in the late time phases.
ISSN:0882-0139
1532-4311
DOI:10.3109/08820130009105144