Effect of 2-Hexenal on the Lipid Peroxidation of Primary Cultured Rat Hepatocytes

Hepatocytes isolated from male Wister rats with or without pre-treatment of phenobarbital (PB), 3-methylcholanthrene (3-MC), β-naphthoflavone (β-NF) and dexamethasone (DMX) by the previous method were cultured in dishes in an atmosphere of 5% CO2-95% air at 37°C. Rat hepatocytes in culture for 24h w...

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Bibliographic Details
Published in:Journal of Health Science 1999/12/31, Vol.45(6), pp.384-390
Main Authors: Hirayama, Teruhisa, Yoshikawa, Akihiro, Kasai, Terue, Watanabe, Tetsushi
Format: Article
Language:eng ; jpn
Subjects:
2-hexenal
lipid peroxidation
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Summary:Hepatocytes isolated from male Wister rats with or without pre-treatment of phenobarbital (PB), 3-methylcholanthrene (3-MC), β-naphthoflavone (β-NF) and dexamethasone (DMX) by the previous method were cultured in dishes in an atmosphere of 5% CO2-95% air at 37°C. Rat hepatocytes in culture for 24h were incubated in Williams'E medium containing 2-hexenal and tert-butylhydroperoxide. Cell membrane oxidation was measured by the fluorometric thiobarbituric acid (TBA) method. Cell membrane oxidation induced by 2-hexenal and tert-butylhydroperoxide and inhibited by the addition of some antioxdants, was only observed in whole hepatocytes treated with 2-hexenal, but not in the purified cell membrane under the same conditions. Therefore, it is assumed that 2-hexenal converted to active substances could injure the membrane of hepatocytes. In PB pretreated-rat hepatocytes, increasing TBA-RS formation was observed. Finally, it is concluded that 2-hexenal is activated in hepatocytes by CYP2B, and that the membrane lipid peroxidation is caused by active compounds such as radicals.
ISSN:1344-9702
1347-5207
DOI:10.1248/jhs.45.384