Effect of millimeter waves on cyclophosphamide induced suppression of the immune system

• Published in: Bioelectromagnetics 2002-12, Vol.23 (8), p.614-621
• Main Authors: Logani, Mahendra K., Anga, Altaf, Szabo, Imre, Agelan, Alexis, Irizarry, Armando R., Ziskin, Marvin C.
• Format: Article
• Language: English
• Subjects: Administration, Topical; Animals; Body Weight - drug effects; Body Weight - radiation effects; Bone Marrow Cells - drug effects; Bone Marrow Cells - radiation effects; Cyclophosphamide - administration & dosage; delayed type hypersensitivity; Dinitrochlorobenzene - administration & dosage; Dinitrochlorobenzene - pharmacology; Drug Hypersensitivity - immunology; Ear; Hypersensitivity, Delayed - chemically induced; Hypersensitivity, Delayed - immunology; Immune Tolerance - drug effects; Immune Tolerance - immunology; Immune Tolerance - radiation effects; Immunity, Cellular - drug effects; Immunity, Cellular - radiation effects; Immunosuppression - methods; Injections, Intraperitoneal; Leukocyte Count; Leukocytes - drug effects; Leukocytes - radiation effects; leukopenia; Leukopenia - chemically induced; Male; Mice; Mice, Inbred BALB C; Microwaves; myelosuppression; Nose - immunology; Reference Values; Skin - drug effects; Skin - immunology; Skin - radiation effects; T-cell mediated immunity; T-Lymphocytes, Regulatory - drug effects; T-Lymphocytes, Regulatory - immunology; T-Lymphocytes, Regulatory - radiation effects; Whole-Body Irradiation
• ISSN: 0197-8462; 1521-186X
• DOI: 10.1002/bem.10058

The effect of millimeter electromagnetic waves (MWs) on cyclophosphamide (CPA) induced toxicity to leukocytes, bone marrow cells, and T‐cell‐mediated immunity was examined. For studying the effect of MWs on CPA induced leukopenia and myelosuppression, BALB/C mice were irradiated for 3 days, 30 min each day, prior to administration of CPA (200 mg/kg). MWs were produced with a Russian made YAV‐1 generator. The device produced 42.2 ± 0.2 GHz modulated wave radiation through a 10 mm × 20 mm rectangular output horn. The animals were irradiated on the nose area. Peak SAR and incident power density were measured as 622 ± 100 W/kg and 31 ± 5 mW/cm2, respectively. For studying the effect of MWs on CPA induced suppression of T‐cell mediated immunity, a delayed type hypersensitivity (DTH) assay in mouse skin was used. The DTH reaction in mouse skin was induced by topical application of dinitrochlorobenzene (DNCB) and quantified by measuring the increase in ear thickness and by histological examination. Treatment of animals with CPA significantly (P