Reevaluation and improved design of the TEM cell in vitro exposure unit for replication studies

The transverse electromagnetic (TEM) cell system developed by Litovitz et al. and utilized by Penafiel et al. for the exposure of cells in T25 flasks at 835 MHz has been reevaluated for the purpose of replicating the studies published by Penafiel. The original setup has been reconstructed as closely...

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Bibliographic Details
Published in:Bioelectromagnetics 2005-04, Vol.26 (3), p.215-224
Main Authors: Nikoloski, Neviana, Fröhlich, Jürg, Samaras, Theodoros, Schuderer, Jürgen, Kuster, Niels
Format: Article
Language:English
Subjects:
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Cell Physiological Phenomena - radiation effects
dosimetry
Environmental Exposure - analysis
Equipment Design
Equipment Failure Analysis
exposure apparatus
Radiation Dosage
Radiometry - instrumentation
Radiometry - methods
Reproducibility of Results
SAR
Sensitivity and Specificity
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Summary:The transverse electromagnetic (TEM) cell system developed by Litovitz et al. and utilized by Penafiel et al. for the exposure of cells in T25 flasks at 835 MHz has been reevaluated for the purpose of replicating the studies published by Penafiel. The original setup has been reconstructed as closely as possible, with improvements enabling blinded exposures, forced cooling and better repeatable positioning of the flasks, as well as tight exposure and environmental parameter control. The signal unit can simulate the original signal but also enables various other exposure schemes. The setup has been evaluated for four T25 flasks filled with 5 and 10 ml of cell medium by experimental and numerical means. Comparing E field, SAR and temperature measurements resulted in good agreement:
ISSN:0197-8462
1521-186X
DOI:10.1002/bem.20067