Preparative Dephosphorylation of Calcium Salts of 2'(3")-Mononucleotides with Extracellular Phosphatase from Spicaria violacea

The properties of the phosphatase present in the culture liquid of Spicaria violacea were investigated. Based on these results, a method for preparative dephosphorylation of calcium salts of 2'(3')-mononucleotides was proposed. A 96-98% yield was achieved at a substrate concentration of 10...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2000-01, Vol.36 (1)
Main Authors: Barai, V N, Severina, IA, Kukharskaya, T A, Zinchenko, AI
Format: Article
Language:English
Subjects:
calcium salts
mononucleotides
nucleosides
phosphatase
Spicaria violacea
Online Access:Get full text
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Summary:The properties of the phosphatase present in the culture liquid of Spicaria violacea were investigated. Based on these results, a method for preparative dephosphorylation of calcium salts of 2'(3')-mononucleotides was proposed. A 96-98% yield was achieved at a substrate concentration of 100 mg/ml. Mild quantitative hydrolysis of RNA to nucleosides can be performed by RNA digestion to mononucleotides with Ca super(2+) followed by the proposed dephosphorylation procedure.
ISSN:0003-6838
DOI:10.1007/BF02738125