Sperm chromatin dispersion by formaldehyde in Wistar rats

Formaldehyde (FA) is an environmental xenobiotic, which is genotoxic and carcinogenic to humans and animals; it induces DNA damage, mutations, and clastogenicity during critical cytogenetic events. FA-mediated oxidative stress is an important mechanism that has been associated with the induction of...

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Bibliographic Details
Published in:Genetics and molecular research 2015-09, Vol.14 (3), p.10816-10826
Main Authors: Betancourt-Martínez, N D, Jiménez-Villarreal, J, Carranza-Rosales, P, Guzmán-Delgado, N E, Leyva Orasma, C, Viveros Valdez, E, Morán-Martínez, J
Format: Article
Language:English
Subjects:
Animals
Body Weight - drug effects
Chromatin - drug effects
Chromatin - ultrastructure
DNA Fragmentation - drug effects
Formaldehyde - toxicity
Injections, Intraperitoneal
Male
Mutagens - toxicity
Oxidative Stress
Rats
Rats, Wistar
Seminiferous Tubules - drug effects
Seminiferous Tubules - ultrastructure
Sperm Count
Sperm Motility - drug effects
Spermatogenesis - drug effects
Spermatozoa - drug effects
Spermatozoa - ultrastructure
Testis - drug effects
Testis - ultrastructure
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Summary:Formaldehyde (FA) is an environmental xenobiotic, which is genotoxic and carcinogenic to humans and animals; it induces DNA damage, mutations, and clastogenicity during critical cytogenetic events. FA-mediated oxidative stress is an important mechanism that has been associated with the induction of cytotoxic and genotoxic damage. Therefore, the objective of this study was to evaluate the dispersion of sperm chromatin and reproductive parameters induced by exposure to different concentrations of FA in Wistar rats. Compared to the percentage of sperm with fragmented DNA in the control group (18.10 ± 8.62%), the percentage of sperm with fragmented DNA increased following exposure to 5, 10, and 30 mg FA/kg body weight (29.60 ± 8.44, 85.20 ± 20.94 and 96.0 ± 7.87, respectively; P = 0.0001). Histopathological alterations were evident, especially in the seminiferous tubules. In conclusion, this study provides experimental evidence concerning the genotoxicity of FA, with particular reference to the decreased sperm concentration and motility and increased dispersion of DNA chromatin in rats.
ISSN:1676-5680
1676-5680
DOI:10.4238/2015.September.9.20