Relationship between sperm density, spermatocrit, sperm motility and spawning date in wild and cultured haddock

• Published in: Journal of fish biology 2004-08, Vol.65 (2), p.319-332
• Main Authors: Rideout, R. M., Trippel, E. A., Litvak, M. K.
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; haddock; Melanogrammus aeglefinus; motility; sperm density; sperm quality; spermatocrit; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
• ISSN: 0022-1112; 1095-8649
• DOI: 10.1111/j.0022-1112.2004.00451.x

Semen was collected repeatedly from captive haddock Melanogrammus aeglefinus and the effect of seasonality on various sperm parameters was investigated. No differences in sperm traits were observed for wild and cultured haddock. A highly significant positive relationship existed between spermatocrit and spermatozoa density. A significant increase in mean spermatocrit occurred throughout the spawning season but the amount of variability explained by collection date was low (35·1%) due to variability between males. Each of 10 males sampled repeatedly throughout the spawning season demonstrated an increase in spermatocrit. No relationship existed between spermatocrit and proportion of motile spermatozoa when spermatocrit was ≤70%. Motility was reduced in semen samples with spermatocrits >70%. The proportion of spermatozoa that were motile decreased with time since activation. Some motility was still observed after 60 min in sea water (0·1–15·2%) for sperm collected at all times within the spawning season. Of those spermatozoa that were motile, the proportion that exhibited forward swimming motion decreased and the proportion that had only vibratory movement increased with time post‐activation. The speed of forward swimming spermatozoa showed no significant relationship with spermatocrit at any time between 0 and 60 min after activation. Swimming speed was negatively related to time since activation, decreasing from 174–240 μm s−1 at 0 min to 80–128 μm s−1 at 60 min after activation.