Mixed-feed exponential feeding for fed-batch culture of recombinant methylotrophic yeast

A simple, accurate model capable of predicting cell growth and methanol utilization during the mixed substrate fed-batch fermentation of Mut^sup S^ recombinant Pichia pastoris was developed and was used to design an exponential feeding strategy for mixed substrate fed-batch fermentation at a constan...

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Bibliographic Details
Published in:Biotechnology letters 2000-03, Vol.22 (5), p.341-346
Main Authors: D'ANJOU, M. C, DAUGULIS, A. J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fermentation
Fundamental and applied biological sciences. Psychology
Growth rate
methanol
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microbiology
Pichia pastoris
Yeasts
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Summary:A simple, accurate model capable of predicting cell growth and methanol utilization during the mixed substrate fed-batch fermentation of Mut^sup S^ recombinant Pichia pastoris was developed and was used to design an exponential feeding strategy for mixed substrate fed-batch fermentation at a constant specific growth rate. Mixed substrate feeding has been shown to boost productivity in recombinant fed-batch culture of P. pastoris, while fixed growth rate exponential feeding during fed-batch culture is a useful tool in process optimization and control.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005612415737