Stabilization of invertase by modification of sugar chains with chitosan

Chitosan was linked to invertase by covalent conjugation to periodate-activated carbohydrate moieties of the enzyme. The thermostability of modified enzyme was enhanced by about 10°C. The half-life at 65°C was increased from 5 min to 5 h. The enzyme stability was enhanced by 20% at pH below 3.0. The...

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Bibliographic Details
Published in:Biotechnology letters 2000-03, Vol.22 (5), p.347-350
Main Authors: GOMEZ, L, RAMIREZ, H. L, VILLALONGA, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Carbohydrates
Chemical synthesis for preparing modified enzymes, enzyme fragments and enzyme analogs
chitosan
Enzyme engineering
Enzymes
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Production of selected enzymes
Protein engineering
Systems stability
Thermodynamics
Urea
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Summary:Chitosan was linked to invertase by covalent conjugation to periodate-activated carbohydrate moieties of the enzyme. The thermostability of modified enzyme was enhanced by about 10°C. The half-life at 65°C was increased from 5 min to 5 h. The enzyme stability was enhanced by 20% at pH below 3.0. The half-life of denaturation by 6 M urea was increased by 2 h.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005664432575