Propachlor removal by Pseudomonas strain GCH1 in an immobilized-cell system

A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-i...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2000-03, Vol.66 (3), p.1190-1194
Main Authors: Martin, M, Mengs, G, Plaza, E, Garbi, C, Sanchez, M, Gibbelo, A, Gutierrez, F, Ferrer, E
Format: Article
Language:English
Subjects:
Acetamides
Acetanilides - metabolism
Bacteria
biodegradation
Biodegradation of pollutants
Biodegradation, Environmental
Biological and medical sciences
Bioreactors
Biotechnology
Cells, Immobilized
Cellular biology
Environment and pollution
Environmental Microbiology and Biodegradation
Environmental Pollutants - metabolism
Flowers & plants
Fundamental and applied biological sciences. Psychology
Herbicides
Herbicides - metabolism
immobilization
Industrial applications and implications. Economical aspects
Propachlor
Pseudomonas
Pseudomonas - cytology
Pseudomonas - metabolism
Soil Microbiology
Soil Pollutants - metabolism
Waste Disposal, Fluid - methods
Water Pollutants, Chemical - metabolism
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Summary:A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide, acetanilide, acetamide, and catechol. Pseudomonas strain GCH1 grew on propachlor with a generation time of 4.2 h and a rate of substrate utilization of 1.75 +/- 0.15 micromol h(-1). Gene expression did not require induction but was subject to catabolite expression. Acetanilide was a growth substrate with a yield of 0.56 +/- 0.02 mg of protein micromol(-1). GCH1 strain cells were immobilized by adsorption onto a ceramic support and were used as biocatalysts in an immobilized cell system. Propachlor elimination reached 98%, with a retention time of 3 h and an initial organic load of 0.5 mM propachlor. The viability of immobilized cells increased 34-fold after 120 days of bioreactor operation.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.66.3.1190-1194.2000