MADS1, a novel MADS-box protein, is involved in the response of Nicotiana benthamiana to bacterial harpin(Xoo)

MADS-box transcription factor genes are well known for their role in floral organ and seed development. In this study, a novel MADS-box-containing gene, designated NbMADS1, was isolated from leaves of Nicotiana benthamiana. The full-length cDNA was 666 bp and encoded a putative polypeptide of 221 aa...

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Bibliographic Details
Published in:Journal of experimental botany 2016-01, Vol.67 (1), p.131-141
Main Authors: Zhang, Huajian, Teng, Wenjun, Liang, Jingang, Liu, Xinyu, Zhang, Haifeng, Zhang, Zhengguang, Zheng, Xiaobo
Format: Article
Language:English
Subjects:
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - metabolism
Cell Death
DNA, Complementary - genetics
DNA, Complementary - metabolism
MADS Domain Proteins - genetics
MADS Domain Proteins - metabolism
Molecular Sequence Data
Nicotiana - genetics
Nicotiana - immunology
Nicotiana - metabolism
Nicotiana - microbiology
Phylogeny
Phytophthora - physiology
Plant Immunity
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Stomata - physiology
Sequence Analysis, DNA
Xanthomonas - genetics
Xanthomonas - physiology
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Summary:MADS-box transcription factor genes are well known for their role in floral organ and seed development. In this study, a novel MADS-box-containing gene, designated NbMADS1, was isolated from leaves of Nicotiana benthamiana. The full-length cDNA was 666 bp and encoded a putative polypeptide of 221 aa with a mass of 24.3 kDa. To assess the role of NbMADS1 in the defence response to bacterial harpin(Xoo), an elicitor of the hypersensitive response, a loss-of-function experiment was performed in N. benthamiana plants using virus-induced gene silencing. Analyses of electrolyte leakage revealed more extensive cell death in the control plants than in NbMADS1-silenced plants. The NbMADS1-silenced plants showed impaired harpin(Xoo)-induced stomatal closure, decreased harpin(Xoo)-induced production of hydrogen peroxide (H2O2) and nitric oxide (NO) in guard cells, and reduced harpin(Xoo)-induced resistance to Phytophthora nicotianae. The compromised stomatal closure observed in the NbMADS1-silenced plants was inhibited by the application of H2O2 and sodium nitroprusside (an NO donor). Taken together, these results demonstrate that the NbMADS1-H2O2-NO pathway mediates multiple harpin(Xoo)-triggered responses, including stomatal closure, hypersensitive cell death, and defence-related gene expression, suggesting that NbMADS1 plays an important role in regulating the response to harpin(Xoo) in N. benthamiana plants.
ISSN:1460-2431
DOI:10.1093/jxb/erv448