Purification and characterization of two chitinases from Streptomyces albovinaceus S-22

Two chitinases, A and B, were purified from the culture supernatant of Streptomyces albovinaceus S-22 by ammonium sulphate fraction (80%) and Sephadex G-200 gel filtration. Both enzymes had molecular weights estimated to be 43 and 45kDa by SDS polyacrylamide gel electrophoresis. The enzymes were act...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2000-02, Vol.16 (1), p.87-89
Main Authors: El-Sayed, E.S.A, Ezzat, S.M, Ghaly, M.F, Mansour, M, El-Bohey, M.A
Format: Article
Language:English
Subjects:
Ammonium
Bacteria
Biological and medical sciences
Biological Sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Chitin
Chitinase
chitinase A
chitinase B
Enzymes
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
Miscellaneous
Mission oriented research
plant health
plant pathogenic fungi
Streptomyces albovinaceus
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Summary:Two chitinases, A and B, were purified from the culture supernatant of Streptomyces albovinaceus S-22 by ammonium sulphate fraction (80%) and Sephadex G-200 gel filtration. Both enzymes had molecular weights estimated to be 43 and 45kDa by SDS polyacrylamide gel electrophoresis. The enzymes were active at 40°C and pH 5.6 after 120min, and stable at temperatures below 40°C in the absence of chitin. The purified enzyme had potential for cell wall lysis of fungal pathogenesis tested.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1023/A:1008926214392