Isolation and characterization of arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii

Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-conta...

Full description

Saved in:
Bibliographic Details
Published in:Plant and cell physiology 2000-01, Vol.41 (1), p.77-83
Main Authors: Fujiwara, S. (Tokyo Univ. of Pharmacy and Life Science, Hachioji (Japan)), Kobayashi, I, Hoshino, S, Kaise, T, Shimogawara, K, Usuda, H, Tsuzuki, M
Format: Article
Language:English
Subjects:
Animals
arsenate
Arsenate-resistant
Arsenate-sensitive
Arsenates - metabolism
Arsenates - pharmacology
ARSENIC COMPOUNDS
BIOLOGICAL PROPERTIES
Biomethylation
Cell Division - drug effects
Cell Division - genetics
Cell Extracts - chemistry
CHLAMYDOMONAS REINHARDTII
Chlamydomonas reinhardtii - drug effects
Chlamydomonas reinhardtii - genetics
Chlamydomonas reinhardtii - isolation & purification
Chromatography, High Pressure Liquid
COMPOSE ARSENICAL
COMPUESTOS ARSENICALES
Dimethylarsinic acid
Drug Resistance
Freshwater
Mutagenesis, Insertional
MUTANT
MUTANTES
MUTANTS
Mutation
Phosphates - metabolism
PROPIEDADES BIOLOGICAS
PROPRIETE BIOLOGIQUE
random insertional mutagenesis
RESISTANCE AUX FACTEURS NUISIBLES
RESISTANCE TO INJURIOUS FACTORS
RESISTENCIA A AGENTES DANINOS
Time Factors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-containing medium were determined with an atomic absorption spectrophotometer. The intracellular levels of arsenic in the arsenate-resistant mutants were all lower than that of the parent strain CC425. Some of the arsenate-sensitive mutants, AS1 and AS3, showed obviously higher levels of arsenic than that of CC425, while other sensitive mutant, AS2, did not accumulate arsenic so much. Analysis of the chemical species of arsenic suggested that inorganic arsenic was converted to dimethylarsinic acid (DMAA) in CC425. However, DMAA was hardly detected in AS2. The mechanisms of the resistance to arsenate are discussed on its uptake and detoxification.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/41.1.77