Determination of Methamphetamine in Human Urine by HPLC Using Post-Column Simon's Reaction

An HPLC method using post-column Simon's reaction (SR-HPLC) for the determination of methamphetamine (MA) has been developed. The detection limit was 1ng, which is about 100 times more sensitive than TLC using Simon's reaction (SR-TLC). The calibration curve was linear in the range of 0.1-...

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Bibliographic Details
Published in:Journal of Health Science 2000/02/01, Vol.46(1), pp.16-20
Main Authors: Chinaka, Satoshi, Tanaka, Seishi, Takayama, Nariaki, Ueda, Kazumasa
Format: Article
Language:English
Subjects:
Simon's reaction
urine
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Summary:An HPLC method using post-column Simon's reaction (SR-HPLC) for the determination of methamphetamine (MA) has been developed. The detection limit was 1ng, which is about 100 times more sensitive than TLC using Simon's reaction (SR-TLC). The calibration curve was linear in the range of 0.1-1000μg/ml, and the intermediate precision of within-run and between-run assays was 1.15% and 2.12%, respectively. MA in 13 urine samples was determined by SR-HPLC and GC with flame ionization detection, and these quantitative values were strongly correlated (r2=0.995). MA could be analyzed without interference peaks. The proposed method is highly selective and quantitative.
ISSN:1344-9702
1347-5207
DOI:10.1248/jhs.46.16