Construction of recombinant Escherichia coli strains for production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate)

Plastic wastes constitute a worldwide environmental problem, and the demand for biodegradable plastics has become high. One of the most important characteristics of microbial polyesters is that they are thermoplastic with environmentally degradable properties. In this study, pUC19/PHA was cloned and...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2004, Vol.113-116 (1-3), p.361-372
Main Authors: Law, Kin-Ho, Chan, Pui-Ling, Lau, Wai-Sum, Cheng, Yin-Chung, Leung, Yun-Chung, Lo, Wai-Hung, Lawford, Hugh, Yu, Hoi-Fu
Format: Article
Language:English
Subjects:
Biodegradation, Environmental
Biotechnology - methods
Calorimetry, Differential Scanning
Carbon - chemistry
Chromatography, Gas
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - metabolism
Fermentation
Magnetic Resonance Spectroscopy
Plasmids - metabolism
Plastics
Polyesters - chemistry
Polyesters - metabolism
Temperature
Time Factors
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Summary:Plastic wastes constitute a worldwide environmental problem, and the demand for biodegradable plastics has become high. One of the most important characteristics of microbial polyesters is that they are thermoplastic with environmentally degradable properties. In this study, pUC19/PHA was cloned and transformed into three different Escherichia coli strains. Among the three strains that were successfully expressed in the production of polyhydroxyalkanoates (PHA), E. coli HMS174 had the highest yield in the production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (P[HB-HV]). The cell dry weight and PHA content of recombinant HMS174 reached as high as 10.27 g/L and 43% (w/w), respectively, in fed-batch fermentor culture. The copolymer of PHA, P(HB-HV), was found in the cells, and the biopolymers accumulated were identified and analyzed by gas chromatography, proton nuclear magnetic resonance spectroscopy, and differential scanning calorimetry. We demonstrated clearly that the E. coli host for PHA production has to be carefully selected to obtain a high yield. The results obtained indicated that a superior E. coli with high PHA production can be constructed with a desirable ratio of P(HB-HV), which has potential applications in industry and medicine.
ISSN:0273-2289
0273-2289
DOI:10.1385/ABAB:114:1-3:361