Chromium speciation in human blood samples based on acetyl cysteine by dispersive liquid-liquid biomicroextraction and in-vitro evaluation of acetyl cysteine/cysteine for decreasing of hexavalent chromium concentration

A rapid and efficient method based on ionic liquid dispersive liquid-liquid biomicroextraction (IL-DLLBME) was used for speciation and preconcentration of Chromium (III, VI) in human blood samples before determination by electro-thermal atomic absorption spectrometer (ET-AAS). In this method, 1-hexy...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2016-01, Vol.118, p.1-8
Main Authors: Shirkhanloo, Hamid, Ghazaghi, Mehri, Mousavi, Hassan Z
Format: Article
Language:English
Subjects:
Acetylcysteine - blood
Acetylcysteine - chemistry
Chromium - blood
Chromium - chemistry
Cysteine - blood
Cysteine - chemistry
Drug Evaluation, Preclinical - methods
Environmental Pollutants - blood
Environmental Pollutants - chemistry
Humans
Liquid Phase Microextraction - methods
Prodrugs - analysis
Prodrugs - chemistry
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Summary:A rapid and efficient method based on ionic liquid dispersive liquid-liquid biomicroextraction (IL-DLLBME) was used for speciation and preconcentration of Chromium (III, VI) in human blood samples before determination by electro-thermal atomic absorption spectrometer (ET-AAS). In this method, 1-hexyl-3-methylimidazolium hexafluorophosphate as a ionic liquid was dissolved in acetone as a dispersant solvent and then the binary solution was rapidly injected by a syringe into the blood samples containing Cr(III), which have already complexed by acetyl cysteine (NAC) at optimized pH. Under the optimal conditions, the linear range (LR), limit of detection (LOD) and preconcentration factor (PF) were obtained 0.03-4.4 μg L(-1), 0.005 μg L(-1) and 10 respectively (RSD
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2015.10.018