NMR of (133)Cs(+) in stretched hydrogels: One-dimensional, z- and NOESY spectra, and probing the ion's environment in erythrocytes

(133)Cs nuclear magnetic resonance (NMR) spectroscopy was conducted on (133)Cs(+) in gelatin hydrogels that were either relaxed or stretched. Stretching generated a septet from this spin-7/2 nucleus, and its nuclear magnetic relaxation was studied via z-spectra, and two-dimensional nuclear Overhause...

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Bibliographic Details
Published in:Journal of magnetic resonance (1997) 2015-12, Vol.261, p.110-120
Main Authors: Kuchel, Philip W, Shishmarev, Dmitry, Puckeridge, Max, Levitt, Malcolm H, Naumann, Christoph, Chapman, Bogdan E
Format: Article
Language:English
Subjects:
Algorithms
Cesium - blood
Cesium Isotopes
Chlorides - blood
Computer Simulation
Erythrocytes - chemistry
Erythrocytes - ultrastructure
Humans
Hydrogels - chemistry
Ions
Microscopy, Interference
Monte Carlo Method
Nuclear Magnetic Resonance, Biomolecular - methods
Software
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Summary:(133)Cs nuclear magnetic resonance (NMR) spectroscopy was conducted on (133)Cs(+) in gelatin hydrogels that were either relaxed or stretched. Stretching generated a septet from this spin-7/2 nucleus, and its nuclear magnetic relaxation was studied via z-spectra, and two-dimensional nuclear Overhauser (NOESY) spectroscopy. Various spectral features were well simulated by using Mathematica and the software package SpinDynamica. Spectra of CsCl in suspensions of human erythrocytes embedded in gelatin gel showed separation of the resonances from the cation inside and outside the cells. Upon stretching the sample, the extracellular (133)Cs(+) signal split into a septet, while the intracellular peak was unchanged, revealing different alignment/ordering properties of the environment inside and around the cells. Differential interference contrast light microscopy confirmed that the cells were stretched when the overall sample was elongated. Analysis of the various spectral features of (133)Cs(+) reported here opens up applications of this K(+) congener for studies of cation-handling by metabolically-active cells and tissues in aligned states.
ISSN:1096-0856
DOI:10.1016/j.jmr.2015.10.011