Effects of copper, hypoxia and acute temperature shifts on mitochondrial oxidation in rainbow trout (Oncorhynchus mykiss) acclimated to warm temperature

• Published in: Aquatic toxicology 2015-12, Vol.169, p.46-57
• Main Authors: Sappal, Ravinder, Fast, Mark, Stevens, Don, Kibenge, Fred, Siah, Ahmed, Kamunde, Collins
• Format: Article
• Language: English
• Subjects: Acclimatization - drug effects; Acclimatization - physiology; Animals; Cold Temperature; Copper; Copper - toxicity; Electron Transport; Electron Transport Complex IV - metabolism; Electron transport system; Energy homeostasis; Hypoxia; Hypoxia - physiopathology; Interactions; Mitochondria - drug effects; Mitochondria - metabolism; Oncorhynchus mykiss; Oncorhynchus mykiss - metabolism; Oncorhynchus mykiss - physiology; Oxidative Phosphorylation; Temperature; Water Pollutants, Chemical - toxicity
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2015.10.006

•Warm acclimation reduced the electron transport system (ETS) efficiency.•Warm acclimation altered the effects of acute temperature shift, hypoxia and Cu on ETS.•Warm acclimation increased thermal sensitivity of state 3 and reduced that of state 4.•Cu stimulated while hypoxia inhibited ETS respiratory activity.•Interactions of Cu and hypoxia on the ETS and plasma metabolites were antagonistic. Temperature fluctuations, hypoxia and metals pollution frequently occur simultaneously or sequentially in aquatic systems and their interactions may confound interpretation of their biological impacts. With a focus on energy homeostasis, the present study examined how warm acclimation influences the responses and interactions of acute temperature shift, hypoxia and copper (Cu) exposure in fish. Rainbow trout (Oncorhynchus mykiss) were acclimated to cold (11°C; control) and warm (20°C) temperature for 3 weeks followed by exposure to environmentally realistic levels of Cu and hypoxia for 24h. Subsequently, mitochondrial electron transport system (ETS) respiratory activity supported by complexes I–IV (CI–IV), plasma metabolites and condition indices were measured. Warm acclimation reduced fish condition, induced aerobic metabolism and altered the responses of fish to acute temperature shift, hypoxia and Cu. Whereas warm acclimation decelerated the ETS and increased the sensitivity of maximal oxidation rates of the proximal (CI and II) complexes to acute temperature shift, it reduced the thermal sensitivity of state 4 (proton leak). Effects of Cu with and without hypoxia were variable depending on the acclimation status and functional index. Notably, Cu stimulated respiratory activity in the proximal ETS segments, while hypoxia was mostly inhibitory and minimized the stimulatory effect of Cu. The effects of Cu and hypoxia were modified by temperature and showed reciprocal antagonistic interaction on the ETS and plasma metabolites, with modest additive actions limited to CII and IV state 4. Overall, our results indicate that warm acclimation came at a cost of reduced ETS efficiency and increased sensitivity to added stressors.