The value of molecular expression of KIT and KIT ligand analysed using real-time polymerase chain reaction and immunohistochemistry as a prognostic indicator for canine cutaneous mast cell tumours

This study investigated the correlation between KIT gene expression determined by immunohistochemistry and real‐time polymerase chain reaction (RT‐PCR) and the rate of tumour recurrence and tumour‐related deaths in dogs affected with mast cell tumour (MCT). Kaplan–Meier curves were constructed to co...

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Bibliographic Details
Published in:Veterinary & comparative oncology 2015-03, Vol.13 (1), p.1-10
Main Authors: Costa Casagrande, T. A., de Oliveira Barros, L. M., Fukumasu, H., Cogliati, B., Chaible, L. M., Dagli, M. L. Z., Matera, J. M.
Format: Article
Language:English
Subjects:
Animals
Biomarkers, Tumor
c-kit
Dog Diseases - metabolism
Dog Diseases - pathology
Dogs
Gene Expression Regulation, Neoplastic
immunohistochemistry
Immunohistochemistry - veterinary
mastocitoma
Mastocytoma - metabolism
Mastocytoma - pathology
Mastocytoma - veterinary
Proto-Oncogene Proteins c-kit - genetics
Proto-Oncogene Proteins c-kit - metabolism
Real-Time Polymerase Chain Reaction - veterinary
Skin Neoplasms - metabolism
Skin Neoplasms - pathology
Skin Neoplasms - veterinary
Stem Cell Factor - genetics
Stem Cell Factor - metabolism
survival time
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Summary:This study investigated the correlation between KIT gene expression determined by immunohistochemistry and real‐time polymerase chain reaction (RT‐PCR) and the rate of tumour recurrence and tumour‐related deaths in dogs affected with mast cell tumour (MCT). Kaplan–Meier curves were constructed to compare tumour recurrence and tumour‐related death between patients. The log‐rank test was used to check for significant differences between curves. KIT‐I, KIT‐II and KIT‐III staining patterns were observed in 9 (11.11%), 50 (61.73%) and 22 (27.16%) tumours, respectively. Tumour recurrence rates and tumour‐related deaths were not associated with KIT staining patterns (P = 0278, P > 0.05), KIT (P = 0.289, P > 0.05) or KIT ligand (P = 0.106, P > 0.05) gene expression. Despite the lack of association between KIT staining pattern and patient survival time, the results suggest a correlation between aberrant KIT localization and increased proliferative activity of MCTs. RT‐PCR seems to be a sensible method for quantitative detection of KIT gene expression in canine MCT, although expressions levels are not correlated with prognosis.
ISSN:1476-5810
1476-5829
DOI:10.1111/vco.12010