Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains

ABSTRACT Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used. Biofilm formation is believed to be one of the possible causes of relapse because of its...

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Bibliographic Details
Published in:Microbiology and immunology 2015-11, Vol.59 (11), p.653-663
Main Authors: Mongkolrob, Rungrawee, Taweechaisupapong, Suwimol, Tungpradabkul, Sumalee
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
antibiotic resistance
Antibiotics
Bacteriology
biofilm
Biofilms
Biofilms - growth & development
Burkholderia pseudomallei - physiology
Drug Resistance - physiology
exopolysaccharide
gas chromatography-mass spectrometry
Humans
Melioidosis
Mutation
Polysaccharides, Bacterial - chemistry
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Summary:ABSTRACT Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used. Biofilm formation is believed to be one of the possible causes of relapse because of its ability to increase drug resistance. EPS in biofilms have been reported to be related to the limitation of antibiotic penetration in B. pseudomallei. However, the mechanisms by which biofilms restrict the diffusion of antibiotics remain unclear. The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains. CLSM revealed a reduction in exopolysaccharide production and disabled micro‐colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance. Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant. These results indicate that the correlation between these phenomena in the B. pseudomallei biofilm at least results from the exopolysaccharide, which may be under the regulation of bpsI, ppk, or rpoS genes.
ISSN:0385-5600
1348-0421
DOI:10.1111/1348-0421.12331