Agrobacterium arsenijevicii sp. nov., isolated from crown gall tumors on raspberry and cherry plum

Two plant-tumorigenic strains KFB 330T and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on...

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Bibliographic Details
Published in:Systematic and applied microbiology 2015-09, Vol.38 (6), p.373-378
Main Authors: Kuzmanović, Nemanja, Puławska, Joanna, Prokić, Anđelka, Ivanović, Milan, Zlatković, Nevena, Jones, Jeffrey B., Obradović, Aleksa
Format: Article
Language:English
Subjects:
16S rDNA
Agrobacterium
Agrobacterium - chemistry
Agrobacterium - classification
Agrobacterium - genetics
Agrobacterium - isolation & purification
ANI
Bacterial Typing Techniques
Cluster Analysis
Crown gall
Cytosol - chemistry
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Fatty Acids - analysis
Genes, Essential
MLSA
Molecular Sequence Data
Molecular Typing
Phylogeny
Plant Tumors - microbiology
Poland
Prunus
Prunus cerasifera
Prunus domestica - microbiology
RNA, Ribosomal, 16S - genetics
Rubus - microbiology
Rubus idaeus
Sequence Analysis, DNA
Serbia
Taxonomy
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Summary:Two plant-tumorigenic strains KFB 330T and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A. nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD, glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, “A. fabrum” and A. pusense. This was further supported by average nucleotide identity values (
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2015.06.001