Characterization of the complete mitochondrial genomes from Polycladida (Platyhelminthes) using next-generation sequencing

The complete mitochondrial genomes of three polycladids, the acotylean Hoploplana elisabelloi and the cotyleans Enchiridium sp. and Prosthiostomum siphunculus have been assembled with high coverage from Illumina sequencing data. The mt genomes contain 36 genes including 12 of the 13 protein-coding g...

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Bibliographic Details
Published in:Gene 2016-01, Vol.575 (2), p.199-205
Main Authors: Aguado, M. Teresa, Grande, Cristina, Gerth, Michael, Bleidorn, Christoph, Noreña, Carolina
Format: Article
Language:English
Subjects:
Animals
Cotylea
Enchiridium
Genome, Helminth
Genome, Mitochondrial
High-Throughput Nucleotide Sequencing
Hoploplana
Metazoa
Mitochondrial genome
Molecular phylogenetics
Neodermata
Phylogeny
Platyhelminthes
Platyhelminths - genetics
Polycladida
Prosthiostomum siphunculus
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Summary:The complete mitochondrial genomes of three polycladids, the acotylean Hoploplana elisabelloi and the cotyleans Enchiridium sp. and Prosthiostomum siphunculus have been assembled with high coverage from Illumina sequencing data. The mt genomes contain 36 genes including 12 of the 13 protein-coding genes characteristic for metazoan mitochondrial genomes, two ribosomal RNA genes, and 22 transfer RNA genes. Gene annotation, gene order, genetic code, start and stop codons and codon bias have been identified. In comparison with the well investigated parasitic Neodermata, our analysis reveals a great diversity of gene orders within Polycladida and Platyhelminthes in general. By analyzing representative genomes of the main groups of Platyhelminthes we explored the phylogenetic relationships of this group. The phylogenetic analyses strongly supported the monophyly of Polycladida, and based on a small taxon sampling suggest the monophyly of Acotylea and Cotylea. •We provide three complete mitochondrial genomes of Polycladida (Platyhelminthes).•Our analysis reveals a great diversity of gene orders within Platyhelminthes.•The phylogenetic analyses strongly supported the monophyly of Polycladida.•The results suggest the monophyly of the two suborders within Polycladida: Acotylea and Cotylea.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2015.08.054