Detection of cytoplasmic proteins from Helicobacter pylori in Colony Lift Immunoassay

Use of the Colony Lift Immunoassay has been described for several Gram negative bacteria of medical interest. In all cases detection was limited to the use of antibodies against outer membrane proteins. Here we describe the adaptation of this method for detection of the cytoplasmic CagA toxin from H...

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Bibliographic Details
Published in:Journal of microbiological methods 2015-12, Vol.119, p.145-146
Main Authors: Rojas-Rengifo, Diana F., Jaramillo, Carlos A., Haas, Rainer, Jiménez-Soto, Luisa F.
Format: Article
Language:English
Subjects:
Bacterial Proteins - analysis
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
CLI
Colony blot
Cytoplasm - chemistry
Cytoplasm - genetics
Cytoplasm - metabolism
Cytoplasmic proteins
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - chemistry
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Helicobacter pylori - metabolism
Humans
Immunoassay
Immunoassay - methods
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Summary:Use of the Colony Lift Immunoassay has been described for several Gram negative bacteria of medical interest. In all cases detection was limited to the use of antibodies against outer membrane proteins. Here we describe the adaptation of this method for detection of the cytoplasmic CagA toxin from Helicobacter pylori. [Display omitted] •Colony Lift Immunoassay for use in Helicobacter pylori•Intracellular protein immunodetection in colony lift assay•Capacity to use membranes for multiple detection of proteins
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2015.10.005