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Induction of secondary carotenogenesis in new halophile microalgae from the genus Dunaliella (Chlorophyceae)
We report on the effects of high light irradiance (480 μmol quanta/(m2·s)) and salinity (160 and 200 g/liter NaCl) on culture growth as well as on cell lipid pigment and fatty acid (FA) composition in three novel strains of halophile microalga from the genus Dunaliella . Based on the ITS1–5.8S rRNA–...
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Published in: | Biochemistry (Moscow) 2015-11, Vol.80 (11), p.1508-1513 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | We report on the effects of high light irradiance (480 μmol quanta/(m2·s)) and salinity (160 and 200 g/liter NaCl) on culture growth as well as on cell lipid pigment and fatty acid (FA) composition in three novel strains of halophile microalga from the genus
Dunaliella
. Based on the ITS1–5.8S rRNA–ITS2 sequence and on the capability of accumulation of secondary (uncoupled from the photosynthetic apparatus) β-carotene, the strains
Dunaliella
sp. BS1 and BS2 were identified as
D. salina
and
Dunaliella
sp. R5 as
D. viridis
. Under conditions optimal for growth, chlorophylls and primary carotenoids (mainly lutein) dominated the pigment profile of all investigated strains. The main FA were represented by unsaturated C18 FA typical of thylakoid membrane structural lipids. In all studied cells, stressors caused a decline in chlorophylls and an increase in unsaturated C16 and C18 FA associated with reserve lipids. The carotenogenic species
D. salina
demonstrated 10-fold increase in carotenoids accompanied by a decline in lutein and a drastic increase in β-carotene (up to 75% of total carotenoids). In
D. viridis
, only 1.5-fold increase in carotenoid content took place, the ratio of major carotenoids remaining essentially unchanged. The role of the carotenogenic response in mechanisms of protection against photooxidative damage is discussed in view of halophile microalgae stress tolerance and application of the new
Dunaliella
strains for biotechnological production of β-carotene. |
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ISSN: | 0006-2979 1608-3040 |
DOI: | 10.1134/S0006297915110139 |